![P-1 [P1]](/static/images/cells-no_picture.jpg)
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平臺編號：bio-68419
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國際編號：CRL-2588?
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細(xì)胞信息： P-1 [P1]
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
P-1 [P1]
是否是腫瘤細(xì)胞：0
物種來源：小鼠
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1 kappa , IgG1; kappa light chain
運輸方式：凍存運輸
品系：BALB/c
ATCC Number：CRL-2588?
生長狀態(tài)：懸浮生長
數(shù)量：大量
器官來源：脾
規(guī)格：0.1ml Designations: P-1 [P1]
Depositors: ?R Lane, RL Mellgren
Isotype: IgG1 kappa , IgG1; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Strain: BALB/c
Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the small subunit of calpain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1986
Comments:Animals were immunized with purified bovine myocardial calpain 2 [53528 ]
Spleen cells were fused with FOX-NY myeloma cells. [53528 ]
The antibody reacts with the small subunit of bovine mu-caplain and m-calpain.
It cross-reacts with the small subunit of human and canine mu-caplain and m-calpain.
The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the small subunit of calpain.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(4) viable cells/ml.
Interval: Maintain cell concentration between 3 X 10(4) and 3 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 24 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53528: Mellgren RL, Lane RD. Myocardial calpain 2 is inhibited by monoclonal antibodies specific for the small, noncatalytic subunit. Biochim. Biophys. Acta 954: 154-160, 1988. PubMed: 2835105
53531: Zhang W, Mellgren RL. Calpain Subunits Remain Associated during Catalysis. Biochem. Biophys. Res. Commun. 227: 890-896, 1996. PubMed: 8954990
53533: Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541
物種來源：小鼠
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1 kappa , IgG1; kappa light chain
運輸方式：凍存運輸
品系：BALB/c
ATCC Number：CRL-2588?
生長狀態(tài)：懸浮生長
數(shù)量：大量
器官來源：脾
規(guī)格：0.1ml Designations: P-1 [P1]
Depositors: ?R Lane, RL Mellgren
Isotype: IgG1 kappa , IgG1; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Strain: BALB/c
Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the small subunit of calpain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1986
Comments:Animals were immunized with purified bovine myocardial calpain 2 [53528 ]
Spleen cells were fused with FOX-NY myeloma cells. [53528 ]
The antibody reacts with the small subunit of bovine mu-caplain and m-calpain.
It cross-reacts with the small subunit of human and canine mu-caplain and m-calpain.
The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the small subunit of calpain.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(4) viable cells/ml.
Interval: Maintain cell concentration between 3 X 10(4) and 3 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 24 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53528: Mellgren RL, Lane RD. Myocardial calpain 2 is inhibited by monoclonal antibodies specific for the small, noncatalytic subunit. Biochim. Biophys. Acta 954: 154-160, 1988. PubMed: 2835105
53531: Zhang W, Mellgren RL. Calpain Subunits Remain Associated during Catalysis. Biochem. Biophys. Res. Commun. 227: 890-896, 1996. PubMed: 8954990
53533: Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541
