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CC39
    CC39
  • 平臺編號:bio-68407
  • 國際編號:HB-274?
  • 細胞信息: CC39
  • 規(guī)格:frozen
  • 用途:ATCC原裝細胞
  • 服務費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

ATCC Number:HB-274?
生長狀態(tài):懸浮生長
數(shù)量:大量
運輸方式:凍存運輸
細胞形態(tài):淋巴樣
免疫類型:IgG1
是否是腫瘤細胞:0
物種來源:小鼠
規(guī)格:0.1ml Designations: CC39
Depositors: ?CJ Howard
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against bovine WC1 (BoWC1)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with bovine thymocytes.
Spleen cells were fused with NS1 myeloma cells.
The antibody is specific for bovine WC1.
It is similar in specificity to CC15 (see ATCC HB-265 ), but appears to bind to a different epitope.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22775: Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. PubMed: 1708546
24379: . . Vet. Immunol. Immunopathol. 32: 1993..

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