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平臺(tái)編號(hào)：bio-68393
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國(guó)際編號(hào)：CRL-1895?
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細(xì)胞信息： RK3E
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
RK3E
數(shù)量：大量
器官來(lái)源：腎臟
ATCC Number：CRL-1895?
相關(guān)疾病：正常
年限：7 days
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：Fischer
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
細(xì)胞形態(tài)：上皮樣
規(guī)格：0.5ml Designations: RK3E
Depositors: ?K Kinzler, B Vogelstein, J Ruppert
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Strain: Fischer
Organ: kidney
Disease: normal
Cell Type: E1A immortalized
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Tumorigenic:No
Age: 7 days
Comments:The RK3E cell line was derived by immortalizing rat kidney cells with the E1A oncogene.
The cells can be transformed with the RAS and GLI oncogenes.
The cells have an essentially normal karyotype with the exception of one 5q.
Transformation with RAS or GLI renders the cells tumorigenic.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove old medium, and add fresh 0.25% trypsin, 0.03% EDTA solution. Let sit 2 minutes, remove trypsin and allow the flask to incubate at 37C for 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22382: Kinzler KW, et al. Identification of an amplified, highly expressed gene in a human glioma. Science 236: 70-73, 1987. PubMed: 3563490
22576: Kinzler KW, Vogelstein B. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Mol. Cell. Biol. 10: 634-642, 1990. PubMed: 2105456
24394: Kinzler KW, et al. The GLI gene is a member of the Kruppel family of zinc finger proteins. Nature 332: 371-374, 1988. PubMed: 2832761
器官來(lái)源：腎臟
ATCC Number：CRL-1895?
相關(guān)疾病：正常
年限：7 days
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：Fischer
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
細(xì)胞形態(tài)：上皮樣
規(guī)格：0.5ml Designations: RK3E
Depositors: ?K Kinzler, B Vogelstein, J Ruppert
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Strain: Fischer
Organ: kidney
Disease: normal
Cell Type: E1A immortalized
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Tumorigenic:No
Age: 7 days
Comments:The RK3E cell line was derived by immortalizing rat kidney cells with the E1A oncogene.
The cells can be transformed with the RAS and GLI oncogenes.
The cells have an essentially normal karyotype with the exception of one 5q.
Transformation with RAS or GLI renders the cells tumorigenic.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove old medium, and add fresh 0.25% trypsin, 0.03% EDTA solution. Let sit 2 minutes, remove trypsin and allow the flask to incubate at 37C for 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22382: Kinzler KW, et al. Identification of an amplified, highly expressed gene in a human glioma. Science 236: 70-73, 1987. PubMed: 3563490
22576: Kinzler KW, Vogelstein B. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Mol. Cell. Biol. 10: 634-642, 1990. PubMed: 2105456
24394: Kinzler KW, et al. The GLI gene is a member of the Kruppel family of zinc finger proteins. Nature 332: 371-374, 1988. PubMed: 2832761
