ATCC Number:HB-69?
是否是腫瘤細(xì)胞:0
物種來源:小鼠
數(shù)量:大量
運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG3
生長狀態(tài):懸浮生長
規(guī)格:0.2ml Designations: MRSS-1 (D2D4)
Depositors: ?BH Hahn, F Ebling
Isotype: IgG3
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against DNA (single stranded)
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Comments:Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells.
The antibody cross-reacts with double stranded DNA and polyinosinic-cytidylic acid (poly I-C).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 58002: Hahn BH, et al. Production of monoclonal murine antibodies to DNA by somatic cell hybrids. Arthritis Rheum. 23: 942-945, 1980. PubMed: 6157395