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MRSS-1 (D2D4)
    MRSS-1 (D2D4)
  • 平臺編號:bio-68387
  • 國際編號:HB-69?
  • 細(xì)胞信息: MRSS-1 (D2D4)
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

ATCC Number:HB-69?
是否是腫瘤細(xì)胞:0
物種來源:小鼠
數(shù)量:大量
運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG3
生長狀態(tài):懸浮生長
規(guī)格:0.2ml Designations: MRSS-1 (D2D4)
Depositors: ?BH Hahn, F Ebling
Isotype: IgG3
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against DNA (single stranded)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Spleen cells from male MRL/1 mice (mice which normally produce antibodies to DNA) were fused with NS-1 myeloma cells.
The antibody cross-reacts with double stranded DNA and polyinosinic-cytidylic acid (poly I-C).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 58002: Hahn BH, et al. Production of monoclonal murine antibodies to DNA by somatic cell hybrids. Arthritis Rheum. 23: 942-945, 1980. PubMed: 6157395

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