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平臺編號：bio-68370
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國際編號：CRL-2024?
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細胞信息： GR-20
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
GR-20
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：大鼠
數(shù)量：大量
生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
免疫類型：IgG2a; kappa light chain
ATCC Number：CRL-2024?
規(guī)格：0.5mg Designations: GR-20
Depositors: ?SW Russell
Isotype: IgG2a; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse receptor for gamma interferon
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with mouse gamma interferon receptor protein purified from solubilized WEHI-3 cells (see ATCC TIB-68 ).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an extracellular epitope on the binding site of the mouse receptor for gamma interferon with an avidity of 3.33 X 10 exp-3 l/mol.
It blocks both interferon - receptor binding, and induction of macrophage mediated tumor cell killing.
It does not have agonist activity in the induction of macrophage killing.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22141: Basu M, et al. A monoclonal antibody against the ligand binding site of the receptor for mouse interferon-gamma. J. Interferon Res. 9: 551-562, 1989. PubMed: 2477471
22295: Basu M, et al. Purification and partial characterization of a receptor protein for mouse interferon gamma. Proc. Natl. Acad. Sci. USA 85: 6282-6286, 1988. PubMed: 2970637
22785: LeClaire RD, et al. Characterization and use of monoclonal and polyclonal antibodies against the mouse interferon-gamma receptor. J. Leukocyte Biol. 51: 507-516, 1992. PubMed: 1376354
是否是腫瘤細胞：0
物種來源：大鼠
數(shù)量：大量
生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
免疫類型：IgG2a; kappa light chain
ATCC Number：CRL-2024?
規(guī)格：0.5mg Designations: GR-20
Depositors: ?SW Russell
Isotype: IgG2a; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse receptor for gamma interferon
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with mouse gamma interferon receptor protein purified from solubilized WEHI-3 cells (see ATCC TIB-68 ).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an extracellular epitope on the binding site of the mouse receptor for gamma interferon with an avidity of 3.33 X 10 exp-3 l/mol.
It blocks both interferon - receptor binding, and induction of macrophage mediated tumor cell killing.
It does not have agonist activity in the induction of macrophage killing.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
- 0.05 mM 2-mercaptoethanol
- fetal bovine serum to a final concentration of 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22141: Basu M, et al. A monoclonal antibody against the ligand binding site of the receptor for mouse interferon-gamma. J. Interferon Res. 9: 551-562, 1989. PubMed: 2477471
22295: Basu M, et al. Purification and partial characterization of a receptor protein for mouse interferon gamma. Proc. Natl. Acad. Sci. USA 85: 6282-6286, 1988. PubMed: 2970637
22785: LeClaire RD, et al. Characterization and use of monoclonal and polyclonal antibodies against the mouse interferon-gamma receptor. J. Leukocyte Biol. 51: 507-516, 1992. PubMed: 1376354
