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平臺(tái)編號(hào)：bio-68367
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國際編號(hào)：CCL-11?
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細(xì)胞信息： NCTC clone 2472
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
NCTC clone 2472
品系：C3H/HeN
數(shù)量：大量
組織來源：subcutaneous connective tissue; areolar and adipose
年限：82 days
ATCC Number：CCL-11?
相關(guān)疾?。赫?br /> 運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：0
物種來源：小鼠
規(guī)格：48T Designations: NCTC clone 2472
Depositors: ?KK Sanford
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/HeN
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 3
Tumorigenic:Yes
Antigen Expression:H-2k
Cytogenetic Analysis:modal number = 52; range = 47 to 173, An extra long telocentric demonstrable in 100/100 cells. No metacentrics were observed.
Age: 82 days
Gender: male
Comments:NCTC clone 2472 was derived in May, 1956, by K.K. Sanford, et al. from NCTC strain 1742 (Subline VII) of the parent NCTC clone 1328. [22230 ]
The parent line was derived by G.D. Likely, et al. in November, 1950, from a culture of subcutaneous areolar and adipose connective tissue taken from a normal 82-day-old male C3H/HeN mouse. [26086 ]
While cultures of NCTC strain 1742 produced sarcomas in 97% of the mice injected, cultures of NCTC clone 2472 under similar conditions produced sarcomas in 65% of the mice injected. [26087 ]
NCTC clone 2472 cells have been cultured since origin in an antibiotic-free mixture of medium NCTC 109 (135), with 10% horse serum.
NCTC clone 2472 has been compared with related clones, such as NCTC clone 2555 (ATCC CCL-12 ), and certain other cell lines with respect to neoplastic and physiologic properties, nutitional needs, karyology, enzyme activities, and rates of glycolysis. [22230 ] [26090 ] [26093 ] [26104 ] [26106 ]
Plating efficiency is approximately 10% in the recommended culture medium.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: NCTC 135 medium, 90%; horse serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 3 times per week
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2040
References: 22230: Sanford KK, et al. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 23: 1035-1059, 1959. PubMed: 14441477
26086: Likely GD, et al. Further studies on the proliferation in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 13: 177-184, 1952. PubMed: 14946507
26087: Sanford KK, et al. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro. J. Natl. Cancer Inst. 15: 215-237, 1954. PubMed: 13233880
26090: Sanford KK. Clonal studies on normal cells and on their neoplastic transformation in vitro. Cancer Res. 18: 747-752, 1958. PubMed: 13573337
26093: Sanford KK, et al. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell. J. Natl. Cancer Inst. 20: 121-145, 1958. PubMed: 13502776
26104: Scott DB, et al. Analysis of enzymatic activities of clones derived from variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 25: 1365-1379, 1960. PubMed: 13749340
26106: . . Exp. Cell Res. 28: 381, 1962.
數(shù)量：大量
組織來源：subcutaneous connective tissue; areolar and adipose
年限：82 days
ATCC Number：CCL-11?
相關(guān)疾?。赫?br /> 運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：0
物種來源：小鼠
規(guī)格：48T Designations: NCTC clone 2472
Depositors: ?KK Sanford
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/HeN
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 3
Tumorigenic:Yes
Antigen Expression:H-2k
Cytogenetic Analysis:modal number = 52; range = 47 to 173, An extra long telocentric demonstrable in 100/100 cells. No metacentrics were observed.
Age: 82 days
Gender: male
Comments:NCTC clone 2472 was derived in May, 1956, by K.K. Sanford, et al. from NCTC strain 1742 (Subline VII) of the parent NCTC clone 1328. [22230 ]
The parent line was derived by G.D. Likely, et al. in November, 1950, from a culture of subcutaneous areolar and adipose connective tissue taken from a normal 82-day-old male C3H/HeN mouse. [26086 ]
While cultures of NCTC strain 1742 produced sarcomas in 97% of the mice injected, cultures of NCTC clone 2472 under similar conditions produced sarcomas in 65% of the mice injected. [26087 ]
NCTC clone 2472 cells have been cultured since origin in an antibiotic-free mixture of medium NCTC 109 (135), with 10% horse serum.
NCTC clone 2472 has been compared with related clones, such as NCTC clone 2555 (ATCC CCL-12 ), and certain other cell lines with respect to neoplastic and physiologic properties, nutitional needs, karyology, enzyme activities, and rates of glycolysis. [22230 ] [26090 ] [26093 ] [26104 ] [26106 ]
Plating efficiency is approximately 10% in the recommended culture medium.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: NCTC 135 medium, 90%; horse serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 3 times per week
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2040
References: 22230: Sanford KK, et al. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 23: 1035-1059, 1959. PubMed: 14441477
26086: Likely GD, et al. Further studies on the proliferation in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 13: 177-184, 1952. PubMed: 14946507
26087: Sanford KK, et al. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro. J. Natl. Cancer Inst. 15: 215-237, 1954. PubMed: 13233880
26090: Sanford KK. Clonal studies on normal cells and on their neoplastic transformation in vitro. Cancer Res. 18: 747-752, 1958. PubMed: 13573337
26093: Sanford KK, et al. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell. J. Natl. Cancer Inst. 20: 121-145, 1958. PubMed: 13502776
26104: Scott DB, et al. Analysis of enzymatic activities of clones derived from variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 25: 1365-1379, 1960. PubMed: 13749340
26106: . . Exp. Cell Res. 28: 381, 1962.
