ATCC Number：CCL-94?
相關疾?。赫?br />
數(shù)量：大量
是否是腫瘤細胞：0
物種來源：貓
年限：12 weeks
細胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
運輸方式：凍存運輸
器官來源：腎皮質
規(guī)格：0.1ml Designations: CRFK
Depositors: ?RA Van Deusen
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Felis catus
Morphology:epithelial
Source: Organ: kidney, cortex
Disease: normal
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Applications:transfection host
Age: 12 weeks
Gender: female
Comments:The cells are free of bovine viral diarrhea (BVD) virus.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and allow culture to stand at room temperature (or at 37C) until cells detach . Add fresh medium, aspirate and dispense into new flasks.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
References: 26266: Crandell RA, et al. Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176-185, 1973. PubMed: 4130570
26267: Fabricant CG, Rich LJ. Microbial studies of feline urolithiasis. J. Am. Vet. Med. Assoc. 158: 976-980, 1971. PubMed: 4930310
26268: Lee KM, et al. Use of an established cat cell line for investigation and quantitation of feline oncornaviruses. J. Natl. Cancer Inst. 49: 55-60, 1972. PubMed: 4338781
28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321
