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細胞形態(tài)：淋巴樣 

數(shù)量：大量 

是否是腫瘤細胞：0 

物種來源：人 

運輸方式：凍存運輸 

生長狀態(tài)：懸浮生長 

器官來源：骨髓 

年限：53 years 

ATCC Number：CCL-243 

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規(guī)格：0.2ml                                                                                                                 Designations 拉丁名 : K-562 

Depositors:HT Holden 

Biosafety Level 生物安全等級 :1 

Shipped 提供形式 :frozen 

Medium  Serum: See Propagation         

Growth Properties:suspension 

Organism:Homo sapiens 

Morphology:lymphoblast 

Source: 

Organ: bone marrow 

Disease: chronic myelogenous leukemia (CML) 

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 

Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.

Please click here  for information regarding the specific requirements for shipment to your location.

Applications 用途 :transfection host 

Tumorigenic:Yes Antigen        

Expression:CD7 (25%) DNA   

Profile (STR):Amelogenin: X CSF1PO: 9,10 

D13S317: 8 D16S539: 11,12 

D5S818: 11,12  

D7S820: 9,11 THO1: 9.3 

TPOX: 8,9 vWA: 16 

Cytogenetic Analysis:The stemline chromosome number is triploid with the 2S component occurring at 4.2%. 

Fifteen markers (M1 and M(15)) occurred in nearly all S metaphases.

Spontaneous non-specific dicentrics occurred, but rarely.

Unstable markers were also rarely seen. The X was disomic, and N9 was nullisomic. 

Isoenzymes:AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 0 PGM3, 1 

Age:53 years Gender:female 

Comments 注釋 :The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises. 

The cell population has been characterized as highly undifferentiated and of the granulocytic series.

Studies conducted by Anderson, et al., on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. 

The K-562 cell line has attained widespread use as a highly sensitive in vitro target for the natural killer assay. See Pross, et al. for a detailed analysis of the in vitro assay of NK cells including the mathematics of quantitation of NK cell activity. 

K-562 blasts are multipotential,hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic,granulocytic and monocytic series. 

The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler and Golde. Cultures from the ATCC stock have been shown to exhibit this sensitivity for assessing human natural killer activity. 

Karyological tudies on various K-562 sublines have been classified into three groups (A,B,C) by Dimery, et al. The strain   obtained by the ATCC most closely resembles the B population. 

Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined.

The line is EBNA negative. 

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005.

To make the complete growth medium, add the following components to the base medium:  fetal bovine serum to a final concentration of 10%. 

Atmosphere 需氧情況 : air, 95%; carbon dioxide (CO2), 5% 

Temperature 培養(yǎng)溫度 : 37.0℃

Subculturing: Protocol: Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml. Subculture at 1 X 10 exp6 cells/ml. 

Medium Renewal: Every 2 to 3 days 

Preservation:

Freeze medium: Complete growth medium 95%;DMSO, 5% 

Storage temperature 保藏溫度 : liquid nitrogen vapor temperature 

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 purified DNA:ATCC CCL-243D purified RNA:ATCC CCL-243R 

References 參考文獻 :867: Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review.Blood 56: 344-350, 1980.PubMed: 6996765 

1101: Ortaldo JR, et al. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line.  J. Natl. Cancer Inst.59: 77-82, 1977. PubMed: 69036 

22609: Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood 45: 321-334, 1975. PubMed: 163658 

26059: Lozzio BB, Lozzio CB. Properties and usefulness of the original K-562 human myelogenous leukemia cell line.Leuk. Res. 3: 363-370, 1979. PubMed: 95026 

26060: Andersson LC, et al. K562--a human erythroleukemic cell line.Int. J. Cancer 23: 143-147, 1979.PubMed: 367973 

26061: Lozzio BB, et al. A multipotential leukemia cell line (K-562) of human origin.  Proc. Soc. Exp.Biol. Med. 166: 546-550, 1981. PubMed: 7194480 

26063: Dimery IW, et al. Variation amongst K562 cell cultures.  Exp. Hematol. 11: 601-610, 1983.PubMed: 6576909 

32357: Chan YJ, et al. Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus.J. Virol. 70:5312-5328,1996. PubMed: 8764042 

32396: Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule.Cell 86: 233-242, 1996.PubMed: 8706128 

32446: Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation.J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663 

32561: Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron.J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842 

32704: Chan YJ, et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus.J. Virol. 70: 8590-8605, 1996. PubMed: 8970984 

33044: Nauseel WM, et al. Effect of the R569W missense mutation on the biosynthesis of myeloperoxidase.J. Biol. Chem. 271: 9546-9549, 1996. PubMed:8621627 

33174: Grune T, et al. Degradation of oxidized proteins in K562 human hematopoietic cells by proteasome.J. Biol. Chem. 271: 15504-15509, 1996. PubMed: 8663134 

48829: Jondal M, Pross H. Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations.Int. J. Cancer 15: 596-605, 1975. PubMed: 806545 

48830: West WH, et al. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line:characterization of effector cells.J. Immunol. 118: 355-361,1977. PubMed: 299761 

48833: Pross HF, et al. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells.IX. The quantitation of natural killer cell activity.J.Clin. Immunol. 1: 51-63, 1981. PubMed: 7334070 

61237: Chen TR. Modal karyotype of human leukemia cell line, K562 (ATCC CCL 243).Cancer Genet.Cytogenet. 17: 55-60, 1985. PubMed: 3857109 

61327: Wu SQ, et al. Extensive amplification of bcr/abl fusion genes clustered on three marker chromosomes in human leukemic cell line K-56.Leukemia 9: 858-862, 1995. PubMed: 7769849