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平臺(tái)編號(hào)：bio-68345
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國(guó)際編號(hào)：CRL-2531?
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細(xì)胞信息： Clone C
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
Clone C
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：其他
器官來(lái)源：腎皮質(zhì)
細(xì)胞形態(tài)：上皮樣
ATCC Number：CRL-2531?
規(guī)格：0.1ml Designations: Clone C
Depositors: ?Q Al-Awqati
Biosafety Level:2 [Cells contain SV40 viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Oryctolagus cuniculus deposited as rabbit
Morphology:epithelial
Source: Organ: kidney, cortex
Cell Type: intercalated; SV40 large T antigen transfected
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Primary cultures of rabbit, bicarbonate-secreting, intercalated cells were isolated. [49845 ]
Cells were transfected by electroporation with the pZipSVtsA58 plasmid encoding a temperature-sensitive large T antigen of SV40 plus the neomycin resistance gene under the control of an SV40 promoter. [49846 ]
Following transfection, a line of G418 resistant cells was obtained and designated IC250. These cells were subcloned (Clone C) and characterized. [49846 ]
The cells divide continuously at the permissive temperature of 32C. At the restrictive temperature of 40C, they cease dividing and assume morphological and transport properties oftrue bicarbonate-secreting intercalated epithelia cells. [49846 ]
They express appropriate ultrastructural features, bind peanut lectin in an apical pattern, are rich in carbonic anhydrase and stain for proton-adenosinetriphosphatase in a basolateral pattern. [49846 ]
These cells reverse the polarity of several proteins and show different cytoskeltal organization depending on the seeding density. [49847 ]
The cell line may be used to study terminal differentiation of intercalated cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
Temperature: 32.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
References: 49845: van Adelsberg J, et al. Isolation and culture of HCO3- -secreting intercalated cells. Am. J. Physiol. 256: C1004-C1011, 1989. PubMed: 2541617
49846: Edwards JC, et al. Conditional immortalization of bicarbonate-secreting intercalated cells from rabbit. Am. J. Physiol. 263: C521-C529, 1992. PubMed: 1325122
49847: van Adelsberg J, et al. An induced extracellular matrix protein reverses the polarity of band 3 in intercalated epithelial cells. Cell 76: 1053-1061, 1994. PubMed: 8137422
51589: Vijayakumar S, et al. Hensin remodels the apical cytoskeleton and induces columnarization of intercalated epithelial cells: processes that resemble terminal differentiation. J. Cell Biol. 144: 1057-1067, 1999. PubMed: 10085301
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：其他
器官來(lái)源：腎皮質(zhì)
細(xì)胞形態(tài)：上皮樣
ATCC Number：CRL-2531?
規(guī)格：0.1ml Designations: Clone C
Depositors: ?Q Al-Awqati
Biosafety Level:2 [Cells contain SV40 viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Oryctolagus cuniculus deposited as rabbit
Morphology:epithelial
Source: Organ: kidney, cortex
Cell Type: intercalated; SV40 large T antigen transfected
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Primary cultures of rabbit, bicarbonate-secreting, intercalated cells were isolated. [49845 ]
Cells were transfected by electroporation with the pZipSVtsA58 plasmid encoding a temperature-sensitive large T antigen of SV40 plus the neomycin resistance gene under the control of an SV40 promoter. [49846 ]
Following transfection, a line of G418 resistant cells was obtained and designated IC250. These cells were subcloned (Clone C) and characterized. [49846 ]
The cells divide continuously at the permissive temperature of 32C. At the restrictive temperature of 40C, they cease dividing and assume morphological and transport properties oftrue bicarbonate-secreting intercalated epithelia cells. [49846 ]
They express appropriate ultrastructural features, bind peanut lectin in an apical pattern, are rich in carbonic anhydrase and stain for proton-adenosinetriphosphatase in a basolateral pattern. [49846 ]
These cells reverse the polarity of several proteins and show different cytoskeltal organization depending on the seeding density. [49847 ]
The cell line may be used to study terminal differentiation of intercalated cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
- 0.005 mg/ml insulin
- 0.005 mg/ml transferrin
- 1.75 ng/ml selenious acid
- 15 ng/ml epidermal growth factor(do not filter)
- 25 ng/ml hydrocortisone
- 10% fetal bovine serum
Temperature: 32.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
References: 49845: van Adelsberg J, et al. Isolation and culture of HCO3- -secreting intercalated cells. Am. J. Physiol. 256: C1004-C1011, 1989. PubMed: 2541617
49846: Edwards JC, et al. Conditional immortalization of bicarbonate-secreting intercalated cells from rabbit. Am. J. Physiol. 263: C521-C529, 1992. PubMed: 1325122
49847: van Adelsberg J, et al. An induced extracellular matrix protein reverses the polarity of band 3 in intercalated epithelial cells. Cell 76: 1053-1061, 1994. PubMed: 8137422
51589: Vijayakumar S, et al. Hensin remodels the apical cytoskeleton and induces columnarization of intercalated epithelial cells: processes that resemble terminal differentiation. J. Cell Biol. 144: 1057-1067, 1999. PubMed: 10085301
