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平臺編號：bio-68341
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國際編號：CRL-2368?
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細(xì)胞信息： 293/CHE-Fc
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
293/CHE-Fc
年限：fetus
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：0
物種來源：人
ATCC Number：CRL-2368?
器官來源：腎臟
規(guī)格：0.5mg Designations: 293/CHE-Fc
Depositors: ?AP Varki
Biosafety Level:2 [CELLS CONTAIN ADENOVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: kidney
Cellular Products:soluble CHE-Fc
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: fetus
Comments:293/CHE-Fc was established by cotransfecting human embryonic 293 cell (ATCC CRL-1573 ) with the plasmid pCHE-Fc and a plasmid encoding Genetic (G418) resistance.
The cell line is stably transfected to secrete the chimeric protein (CHE-Fc), a recombinant soluble chirmeric protein useful as a probe to detect 9-O-Acetylated sialic acids.
CHE-Fc is the fusion protein of hemagglutinin-esterase from Influenza C with human IgG1 Fc.
CHE-Fc is a bifunctional molecule that can be used either to cleave acetyl groups at the ninth position of sialic acids or as a binding probe for 9-O-sialic acids.
293/CHE-Fc cells are G418 resistant.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1 mg/ml G-418, 90%; heat inactivated fetal bovine serum, 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 24 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
parental cell line:ATCC CRL-1573
References: 38258: Klein A, et al. 9-O-Acetylated sialic acids have widespread but selective expression: Analysis using a chimeric dual-function probe derived from influenza C hemaggIutinin-estrase. Proc. Natl. Acad. Sci. USA 91: 7782-7786, 1994. PubMed: 8052660
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：0
物種來源：人
ATCC Number：CRL-2368?
器官來源：腎臟
規(guī)格：0.5mg Designations: 293/CHE-Fc
Depositors: ?AP Varki
Biosafety Level:2 [CELLS CONTAIN ADENOVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: kidney
Cellular Products:soluble CHE-Fc
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: fetus
Comments:293/CHE-Fc was established by cotransfecting human embryonic 293 cell (ATCC CRL-1573 ) with the plasmid pCHE-Fc and a plasmid encoding Genetic (G418) resistance.
The cell line is stably transfected to secrete the chimeric protein (CHE-Fc), a recombinant soluble chirmeric protein useful as a probe to detect 9-O-Acetylated sialic acids.
CHE-Fc is the fusion protein of hemagglutinin-esterase from Influenza C with human IgG1 Fc.
CHE-Fc is a bifunctional molecule that can be used either to cleave acetyl groups at the ninth position of sialic acids or as a binding probe for 9-O-sialic acids.
293/CHE-Fc cells are G418 resistant.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1 mg/ml G-418, 90%; heat inactivated fetal bovine serum, 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 24 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
parental cell line:ATCC CRL-1573
References: 38258: Klein A, et al. 9-O-Acetylated sialic acids have widespread but selective expression: Analysis using a chimeric dual-function probe derived from influenza C hemaggIutinin-estrase. Proc. Natl. Acad. Sci. USA 91: 7782-7786, 1994. PubMed: 8052660
