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平臺編號：bio-68334
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國際編號：CRL-2424?
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細(xì)胞信息： C305
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
C305
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-2424?
數(shù)量：大量
生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
是否是腫瘤細(xì)胞：0
物種來源：小鼠
免疫類型：IgM kappa
規(guī)格：0.1ml Designations: C305
Depositors: ?A Weiss
Isotype: IgM kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphoblast;
Cellular Products:immunoglobulin; monoclonal antibody; against the beta chain of the T cell antigen receptor on Jurkat cells
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with cells from the human T cell line, Jurkat, E6-1 (ATCC TIB-152 ).Spleen cells were fused with Sp2/0 myeloma cells.The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells.It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 23436: Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306
39758: Goldsmith MA, Weiss A. Early signal transduction by the antigen receptor without commitment to T cell activation. Science 240: 1029-1031, 1988. PubMed: 3259335
39759: Wu J, et al. Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation. Immunity 4: 593-602, 1996. PubMed: 8673706
ATCC Number：CRL-2424?
數(shù)量：大量
生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
是否是腫瘤細(xì)胞：0
物種來源：小鼠
免疫類型：IgM kappa
規(guī)格：0.1ml Designations: C305
Depositors: ?A Weiss
Isotype: IgM kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphoblast;
Cellular Products:immunoglobulin; monoclonal antibody; against the beta chain of the T cell antigen receptor on Jurkat cells
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with cells from the human T cell line, Jurkat, E6-1 (ATCC TIB-152 ).Spleen cells were fused with Sp2/0 myeloma cells.The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells.It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 23436: Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306
39758: Goldsmith MA, Weiss A. Early signal transduction by the antigen receptor without commitment to T cell activation. Science 240: 1029-1031, 1988. PubMed: 3259335
39759: Wu J, et al. Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation. Immunity 4: 593-602, 1996. PubMed: 8673706
