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年限：10 weeks old
ATCC Number：CRL-2456?
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：?jiǎn)魏思?xì)胞/巨噬細(xì)胞
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
數(shù)量：大量
品系：C57BL/6J
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)，少量貼壁
器官來(lái)源：肺
規(guī)格：0.5mg Designations: AMJ2-C11
Depositors: ?AV Palleroni
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, with some loosely adherent cells
Organism: Mus musculus deposited as mouse
Morphology:macrophage


Source: Organ: lung
Strain: C57BL/6J
Cell Type: macrophage (alveolar); infected with J2 virus
Cellular Products:interleukin-6 (interleukin 6, IL-6)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 -
Age: 10 weeks old
Gender: female
Comments:AMJ2-C8 (ATCC CRL-2455 ) and AMJ2-C11 (ATCC CRL-2456 ) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. [48933 ]
Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines.
Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators.
AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP).
AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP.
The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors.
Constitutive expression of MHC-class-II antigens was low on AMJ2-C11 but was increased following exposure to rMuIFN-gamma.
The cell line did not secrete substantial amounts of IL-1 or TNF but did secrete large amounts of IL-6.
The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS. [48934 ]
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Firmly tap the flask against palm of hand to dislodge any attached cells and transfer along with the floating cells into new flasks.
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 48933: Palleroni AV, et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973
48934: Palleroni AV, et al. Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450