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平臺(tái)編號(hào)：bio-68312
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國際編號(hào)：CRL-8164?
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細(xì)胞信息： BF-11
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
BF-11
生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2a
ATCC Number：CRL-8164?
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：褐鼠
規(guī)格：0.1ml Designations: BF-11
Depositors: ?University Patents, Inc.
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human erythropoietin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human urinary erythropoietin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds alpha, beta and asialo forms of human erythropoietin.
The antibody has been given the name Epoclonalan.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1666: Weiss TL, et al. Characterization of a monoclonal antibody to human erythropoietin. Proc. Natl. Acad. Sci. USA 79: 5465-5469, 1982. PubMed: 6957875
3924: Goldwasser E, et al. Monoclonal anti-erythropoietin. US Patent 4,558,005 dated Dec 10 1985
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2a
ATCC Number：CRL-8164?
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：褐鼠
規(guī)格：0.1ml Designations: BF-11
Depositors: ?University Patents, Inc.
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human erythropoietin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human urinary erythropoietin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds alpha, beta and asialo forms of human erythropoietin.
The antibody has been given the name Epoclonalan.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1666: Weiss TL, et al. Characterization of a monoclonal antibody to human erythropoietin. Proc. Natl. Acad. Sci. USA 79: 5465-5469, 1982. PubMed: 6957875
3924: Goldwasser E, et al. Monoclonal anti-erythropoietin. US Patent 4,558,005 dated Dec 10 1985
