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平臺(tái)編號(hào)：bio-68306
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國際編號(hào)：CRL-1821?
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細(xì)胞信息： ES-E14TG2a
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
ES-E14TG2a
數(shù)量：大量
年限：embryo, blastocyst embryo, blastocyst
細(xì)胞類型：其他細(xì)胞類型
品系：129/Ola
是否是腫瘤細(xì)胞：0
物種來源：小鼠
器官來源：胚胎
生長狀態(tài)：貼壁生長
ATCC Number：CRL-1821?
細(xì)胞形態(tài)：其他
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：15次 Designations: ES-E14TG2a
Depositors: ?T Doetschman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:spherical
Source: Organ: embryo
Cell Type: embryonic stem cell , blastocyst derived
Strain: 129/Ola
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Age: embryo, blastocyst embryo, blastocyst
Comments:This is a derivative of one of several embryonal stem cell (ES) lines developed by M. Hooper in 1987.
The cells are deficient in HGPRT (HPRT) and are resistant to 0.06 mM 6-thioguanine.
The cells remain undifferentiated when cultured on feeder layers (embryonic fibroblasts or STO cells).
The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
When injected into blastocysts, the cells can colonize the germline.
After conventional molecular gene modification techniques, they may be used to reconstitute mouse embryos.
They may also be of use in studies of early post implantation structures.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.1 mM ; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution. Allow the trypsin to loosen the cells, add fresh medium, aspirate and dispense into new flasks containing pre-plated irradiated feeder cells.
Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 22254: Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655
22923: Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599
22925: Doetschman T, et al. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574
年限：embryo, blastocyst embryo, blastocyst
細(xì)胞類型：其他細(xì)胞類型
品系：129/Ola
是否是腫瘤細(xì)胞：0
物種來源：小鼠
器官來源：胚胎
生長狀態(tài)：貼壁生長
ATCC Number：CRL-1821?
細(xì)胞形態(tài)：其他
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：15次 Designations: ES-E14TG2a
Depositors: ?T Doetschman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:spherical
Source: Organ: embryo
Cell Type: embryonic stem cell , blastocyst derived
Strain: 129/Ola
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Age: embryo, blastocyst embryo, blastocyst
Comments:This is a derivative of one of several embryonal stem cell (ES) lines developed by M. Hooper in 1987.
The cells are deficient in HGPRT (HPRT) and are resistant to 0.06 mM 6-thioguanine.
The cells remain undifferentiated when cultured on feeder layers (embryonic fibroblasts or STO cells).
The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
When injected into blastocysts, the cells can colonize the germline.
After conventional molecular gene modification techniques, they may be used to reconstitute mouse embryos.
They may also be of use in studies of early post implantation structures.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.1 mM ; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution. Allow the trypsin to loosen the cells, add fresh medium, aspirate and dispense into new flasks containing pre-plated irradiated feeder cells.
Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 22254: Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655
22923: Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599
22925: Doetschman T, et al. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574
