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平臺(tái)編號(hào)：bio-68292
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國(guó)際編號(hào)：ATCCCCL-163.2?
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細(xì)胞信息： Mus musculus, mouse
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
M-MSV-BALB/3T3
品系：BALB/c
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
ATCC Number：CCL-163.2?
數(shù)量：大量
年限：14 to 17 day gestation embryo
細(xì)胞類(lèi)型：其他細(xì)胞類(lèi)型
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
器官來(lái)源：胚胎
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
規(guī)格：0.1ml Designations: M-MSV-BALB/3T3
Depositors: ?S Aaronson
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: BALB/c
Cell Type: fibroblastMoloney murine sarcoma virus (Mo-MSV) transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:H-2d
Age: 14 to 17 day gestation embryo
Comments:Do not produce M-MSV.
Derived from BALB/3T3 (CCL-163).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; bovine calf serum, 10%
Temperature: 37.0℃
Subculturing: Protocol: Remove medium, add fresh 0.1% trypsin for 3 to 5 minutes, remove trypsin and let the culture stand at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Related Products:parental cell line:ATCC CCL-163
References: 22253: Aaronson SA, Rowe SP. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology 42: 9-19, 1970. PubMed: 4318987
