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平臺(tái)編號(hào)：bio-68280
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國(guó)際編號(hào)：TIB-165?
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細(xì)胞信息： 10-1.D.2
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
10-1.D.2
ATCC Number：TIB-165?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
免疫類(lèi)型：IgG2b
規(guī)格：0.5mg Designations: 10-1.D.2
Depositors: ?DE Mosier
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Lyb 2.1 mouse B cell (B lymphocyte) antigen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with spleen cells from DBA/2 mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Lyb-2.1 antigen is a mouse B cell differentiation antigen (45000 dalton glycoprotein).
The antibody induces blastogenesis in B cells, and this response is limited to the Lyb 5+ class of B cells.
B cells activated in this way mature into immunoglobulin secreting cells in the presence of T cell replacing factors (medium from Con A treated T cells).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 968: Subbarao B, Mosier DE. Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody. J. Immunol. 130: 2033-2037, 1983. PubMed: 6601145
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
免疫類(lèi)型：IgG2b
規(guī)格：0.5mg Designations: 10-1.D.2
Depositors: ?DE Mosier
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Lyb 2.1 mouse B cell (B lymphocyte) antigen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with spleen cells from DBA/2 mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Lyb-2.1 antigen is a mouse B cell differentiation antigen (45000 dalton glycoprotein).
The antibody induces blastogenesis in B cells, and this response is limited to the Lyb 5+ class of B cells.
B cells activated in this way mature into immunoglobulin secreting cells in the presence of T cell replacing factors (medium from Con A treated T cells).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 968: Subbarao B, Mosier DE. Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody. J. Immunol. 130: 2033-2037, 1983. PubMed: 6601145
