![R3 [33-10ras3]](/static/images/cells-no_picture.jpg)
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平臺編號：bio-68278
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國際編號：CRL-2764?
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細(xì)胞信息： R3 [33-10ras3]
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
R3 [33-10ras3]
ATCC Number：CRL-2764?
細(xì)胞形態(tài)：神經(jīng)元
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：褐鼠
數(shù)量：大量
規(guī)格：0.5mg Designations: R3 [33-10ras3]
Depositors: ?PI Patel
Biosafety Level:2 [Cells contain SV40 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus
Morphology:neuronal
Source: Cell Type: neuronal Schwann cell; immortalized with SV40 large T antigen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Temperature Effects: Restrictive temperature: 39.5°C yes
Permissive temperature: 33°C yes
Tumorigenic:Yes
Comments:AntigenExp:
SV40-tsA58 large T antigen [PubMed:3049071]Oncogene:
v-Ha-ras[PubMed:3049071]Products:
Myelin-associated glycoprotein (Mag); Protein and mRNA, negative [PubMed: 12210843]Myelin basic protein (Mbp); Protein and mRNA, negative [PubMed: 12210843]Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); Protein and mRNA, negative [PubMed: 12210843]peripheral myelin protein 22 (Pmp22); protein, negative; mRNA, positive; [PubMed: 12210843]S100 calcium-binding protein, beta (neural) (S100b) protein, positive [PubMed: 12210843]Receptors:
Nerve growth factor receptor (Ngfr) Protein and mRNA, negative
Comments:
Primary cells were infected with the recombinant retrovirus LJ-tsSVLT containing the temperature sensitive tsA58 large SV40 T oncogene and a gene encoding G418 resistance. Clones were selected in the presence of 0.4 mg/ml G418 and super-infected with Zipras 6 containing v-Ha-ras at the permissive temperature of 33C [PubMed:3049071]. The cells proliferate at both the permissive temperature of 33C and the restrictive temperature of 39.5C. However, the growth rate is increased and growth factor requirements are reduced at the permissive temperature [PubMed: 3049071]. At the restrictive temperature, expression of large T antigen was not detected [PubMed: 3049071]. Expression of p21-v-Ha-ras was not detected [PubMed: 3049071].
A culture submitted to the ATCC in December of 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 33.0°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 64424: Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843
71896: Ridley AJ, et al. Ras-mediated cell cycle arrest is altered by nuclear oncogenes to induce Schwann cell transformation. EMBO J. 7: 1635-1645, 1988. PubMed: 3049071
細(xì)胞形態(tài)：神經(jīng)元
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：褐鼠
數(shù)量：大量
規(guī)格：0.5mg Designations: R3 [33-10ras3]
Depositors: ?PI Patel
Biosafety Level:2 [Cells contain SV40 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus
Morphology:neuronal
Source: Cell Type: neuronal Schwann cell; immortalized with SV40 large T antigen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Temperature Effects: Restrictive temperature: 39.5°C yes
Permissive temperature: 33°C yes
Tumorigenic:Yes
Comments:AntigenExp:
SV40-tsA58 large T antigen [PubMed:3049071]Oncogene:
v-Ha-ras[PubMed:3049071]Products:
Myelin-associated glycoprotein (Mag); Protein and mRNA, negative [PubMed: 12210843]Myelin basic protein (Mbp); Protein and mRNA, negative [PubMed: 12210843]Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); Protein and mRNA, negative [PubMed: 12210843]peripheral myelin protein 22 (Pmp22); protein, negative; mRNA, positive; [PubMed: 12210843]S100 calcium-binding protein, beta (neural) (S100b) protein, positive [PubMed: 12210843]Receptors:
Nerve growth factor receptor (Ngfr) Protein and mRNA, negative
Comments:
Primary cells were infected with the recombinant retrovirus LJ-tsSVLT containing the temperature sensitive tsA58 large SV40 T oncogene and a gene encoding G418 resistance. Clones were selected in the presence of 0.4 mg/ml G418 and super-infected with Zipras 6 containing v-Ha-ras at the permissive temperature of 33C [PubMed:3049071]. The cells proliferate at both the permissive temperature of 33C and the restrictive temperature of 39.5C. However, the growth rate is increased and growth factor requirements are reduced at the permissive temperature [PubMed: 3049071]. At the restrictive temperature, expression of large T antigen was not detected [PubMed: 3049071]. Expression of p21-v-Ha-ras was not detected [PubMed: 3049071].
A culture submitted to the ATCC in December of 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 33.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 33?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 33?C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 64424: Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843
71896: Ridley AJ, et al. Ras-mediated cell cycle arrest is altered by nuclear oncogenes to induce Schwann cell transformation. EMBO J. 7: 1635-1645, 1988. PubMed: 3049071
