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平臺編號：bio-68274
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國際編號：CRL-2381?
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細(xì)胞信息： JAA-F11
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
JAA-F11
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：CRL-2381?
運輸方式：凍存運輸
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG3; kappa light chain
規(guī)格：0.5mg Designations: JAA-F11
Depositors: ?K Rittenhouse-Diakun
Isotype: IgG3; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphoblast;
Cellular Products:immunoglobulin; monoclonal antibody; against the saccharide antigen, Gal beta1-3GalNAc (T antigen)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:The hybridoma cell line JAA-F11 was established in 1995.
The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen).
The saccharide antigen, Gal beta1-3GalNAc is of biologic importance in many systems.
It is a tumor-associated carbohydrate antigen, a temporally expressed antigen in germinal center B cells and cortical T cells, a parasite-associated antigen, a spermatozoa vitality marker and an antigen on aged red blood cells.
The antibody binds to breast adenocarcinoma tissue but not to normal tissues.
It may be useful in tumor studies, germinal center studies and T cell maturation studies.
The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with: 0.1 mM NEAA, 1.0% MEM Vitamins and 10% fetal bovine serum
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 2 X 10 exp5 and 5 X 10 exp5 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
References: 38875: Rittenhouse-Diakun K, et al. Development and characterization of monoclonal antibody to T-antigen: (gal beta1-3GalNAc-alpha-O). Hybridoma 17: 165-173, 1998. PubMed: 9627057
物種來源：小鼠
ATCC Number：CRL-2381?
運輸方式：凍存運輸
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG3; kappa light chain
規(guī)格：0.5mg Designations: JAA-F11
Depositors: ?K Rittenhouse-Diakun
Isotype: IgG3; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphoblast;
Cellular Products:immunoglobulin; monoclonal antibody; against the saccharide antigen, Gal beta1-3GalNAc (T antigen)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:The hybridoma cell line JAA-F11 was established in 1995.
The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen).
The saccharide antigen, Gal beta1-3GalNAc is of biologic importance in many systems.
It is a tumor-associated carbohydrate antigen, a temporally expressed antigen in germinal center B cells and cortical T cells, a parasite-associated antigen, a spermatozoa vitality marker and an antigen on aged red blood cells.
The antibody binds to breast adenocarcinoma tissue but not to normal tissues.
It may be useful in tumor studies, germinal center studies and T cell maturation studies.
The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with: 0.1 mM NEAA, 1.0% MEM Vitamins and 10% fetal bovine serum
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 2 X 10 exp5 and 5 X 10 exp5 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
References: 38875: Rittenhouse-Diakun K, et al. Development and characterization of monoclonal antibody to T-antigen: (gal beta1-3GalNAc-alpha-O). Hybridoma 17: 165-173, 1998. PubMed: 9627057
