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平臺(tái)編號(hào)：bio-68266
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國際編號(hào)：CRL-2244?
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細(xì)胞信息： pgsD-677
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
pgsD-677
ATCC Number：CRL-2244?
相關(guān)疾病：其他疾病
是否是腫瘤細(xì)胞：0
物種來源：倉鼠
細(xì)胞形態(tài)：上皮樣
數(shù)量：大量
器官來源：卵巢
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.2ml Designations: pgsD-677
Depositors: ?JD Esko
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Cricetulus griseus
Morphology:epithelial
Source: Organ: ovary
Disease: heparin sulfate deficient
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:NOTE: This line is available subject to the following: 1.) The CHO cell mutant was deposited in the ATCC by Dr. Jeffrey D. Esko and is provided for research purposes only as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. The cells are provided with the understanding that they will not be used for commercial purposes. 2.) All products derived from the cells or genetically altered forms of the cells cannot be commercialized without express permission from the University of Alabama at Birmingham. Commercial interests are the exclusive property of the University of Alabama at Birmingham. 3.) Any proposed commercial uses of these cells must first be negotiated with the Research Foundation, University of Alabama at Birmingham, Birmingham, AL 35294. Telephone: (205) 934-9911. 4.) In all papers reporting any use of these cells or derived products, a direct reference will be made to the original publication listed above.
Gender: female
Comments:PgsD-677 is a Chinese hamster ovary cell mutant deficient in the polymerization of heparin sulfate.
The cell line was derived from CHO-K1 cells (see ATCC CCL-61 ) treated with mutagen (ethylmethanesulfonate) and screened for mutants defective in proteoglycan synthesis.
PgsD-677 cells do not produce heparin sulfate.
They lack both N-acetylglucosaminyltransferase and glucuronyltransferase activities that are required for synthesis of heparin sulfate.
They do produce chondroitin sulfate.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Rinse the cell sheet with fresh 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the culture to sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23328: Lidholt K, et al. A single mutation affects both N-acetylglucosaminyltransferase and glucuronosyltransferase activities in a Chinese hamster ovary cell mutant defective in heparan sulfate biosynthesis. Proc. Natl. Acad. Sci. USA 89: 2267-2271, 1992. PubMed: 1532254
23505: Esko JD, et al. Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096, 1988. PubMed: 3137658
相關(guān)疾病：其他疾病
是否是腫瘤細(xì)胞：0
物種來源：倉鼠
細(xì)胞形態(tài)：上皮樣
數(shù)量：大量
器官來源：卵巢
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.2ml Designations: pgsD-677
Depositors: ?JD Esko
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Cricetulus griseus
Morphology:epithelial
Source: Organ: ovary
Disease: heparin sulfate deficient
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:NOTE: This line is available subject to the following: 1.) The CHO cell mutant was deposited in the ATCC by Dr. Jeffrey D. Esko and is provided for research purposes only as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. The cells are provided with the understanding that they will not be used for commercial purposes. 2.) All products derived from the cells or genetically altered forms of the cells cannot be commercialized without express permission from the University of Alabama at Birmingham. Commercial interests are the exclusive property of the University of Alabama at Birmingham. 3.) Any proposed commercial uses of these cells must first be negotiated with the Research Foundation, University of Alabama at Birmingham, Birmingham, AL 35294. Telephone: (205) 934-9911. 4.) In all papers reporting any use of these cells or derived products, a direct reference will be made to the original publication listed above.
Gender: female
Comments:PgsD-677 is a Chinese hamster ovary cell mutant deficient in the polymerization of heparin sulfate.
The cell line was derived from CHO-K1 cells (see ATCC CCL-61 ) treated with mutagen (ethylmethanesulfonate) and screened for mutants defective in proteoglycan synthesis.
PgsD-677 cells do not produce heparin sulfate.
They lack both N-acetylglucosaminyltransferase and glucuronyltransferase activities that are required for synthesis of heparin sulfate.
They do produce chondroitin sulfate.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Rinse the cell sheet with fresh 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the culture to sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23328: Lidholt K, et al. A single mutation affects both N-acetylglucosaminyltransferase and glucuronosyltransferase activities in a Chinese hamster ovary cell mutant defective in heparan sulfate biosynthesis. Proc. Natl. Acad. Sci. USA 89: 2267-2271, 1992. PubMed: 1532254
23505: Esko JD, et al. Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096, 1988. PubMed: 3137658
