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年限：20 years
組織來(lái)源：bronchus
細(xì)胞形態(tài)：上皮樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來(lái)源：肺
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
ATCC Number：CRL-2503?
相關(guān)疾?。赫?br /> 運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
規(guī)格：48T Designations: NL20
Depositors: ?JH Schiller
Biosafety Level:2 [Cells contain SV40 viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:epithelial


Source: Organ: lung
Tissue: bronchus
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:No
Cytogenetic Analysis:near diploid
Age: 20 years
Gender: female
Ethnicity: Caucasian, White
Comments:NL20 (ATCC CRL-2503 ) is an immortalized, nontumorigenic human bronchial epithelial cell line derived from normal bronchus taken from an accident victim at autopsy. [47429 ]
The cell line was established by transfection with the origin of replication-defective SV40 large T plasmid, p129.
NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line.
After three months the tumor was removed and placed in culture. At passage 3, these cells were re-injected into nude mice.
One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. This cell line (ATCC CRL-2504 ) remains tumorigenic up to at least passage 250.
The non-tumorigenic NL20 cell line and the tumorigenic NL20-TA cell line form a pair of immortal cell lines that can be used to study tumor progression.
Propagation: ATCC complete growth medium: Ham's F12 medium with 1.5 g/L sodium bicarbonate, 2.7 g/L glucose, 2.0 mM L-glutamine, 0.1 mM nonessential amino acids, 0.005 mg/ml insulin, 10 ng/ml epidermal growth factor, 0.001 mg/ml transferrin, 500 ng/ml hydrocortisone and 4% fetal bovine serum
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:12 to 1: 20 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add dissociation solution (0.02% EDTA and 5% dialized fetal bovine serum in Ca-Mg free Hanks' BSS) and allow the flask to sit at 37C for 12 minutes or until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subculture weekly.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
derivative:ATCC CRL-2504
References: 47428: Schiller JH, et al. Phenotypic, molecular and genetic characterization of transformed human bronchial epithelial cell strains. Int. J. Oncol. 4: 461-470, 1994.
47429: Schiller JH, Bittner G. Loss of the tumorigenic phenotype with in vitro, but not in vivo, passaging of a novel series of human bronchial epithelial cell lines: possible role of an alpha 5/beta 1-integrin-fibronectin interaction. Cancer Res. : 6215-6221, 1995. PubMed: 8521416
47430: Wittenkeller JL, et al. Comparison of spontaneous and induced mutation rates in animmortalized human bronchial epithelial cell line and its tumorigenic derivative. Oncology 54: 335-341, 1997. PubMed: 9216860
47431: Schiller JH, et al. Karyotypic changes associated with spontaneous acquisition and loss of tumorigenicity in a human transformed bronchial epithelial cell line: evidence for in vivo selection of transformed clones. In Vitro Cell. Dev. Biol. Anim. 34: 283-289, 1998. PubMed: 9590501