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運輸方式：凍存運輸 

ATCC Number：TIB-215 

生長狀態(tài)：懸浮生長 

數(shù)量：大量 

細胞形態(tài)：淋巴樣 

免疫類型：IgM 

細胞類型：B淋巴細胞 

是否是腫瘤細胞：0 

物種來源：人 

規(guī)格：1mg                                                                                                                   Designations 拉丁名 : SKW 6.4          

Depositors:P Ralph         

Isotype: IgM         

Biosafety Level 生物安全等級 :2 [Cells Contain HERPESVIRUS ]         

Shipped 提供形式 :frozen         

Medium Serum: See Propagation         

Growth Properties:suspension          

Organism:Homo sapiens         

Morphology:lymphoblast        

Source:          

Cell Type:Blymphocyte; Epstein-Barr virus (EBV) transformed   

Cellular Products:immunoglobulin; monoclonal antibody         

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the         transfer of this ATCC material. 

Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.

Please click here for information regarding the specific requirements for shipment to your location.DNA Profile (STR):Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 THO1: 8,9.3 TPOX: 8,11           vWA: 17,20   

Comments 注釋 :The cells respond to T Cell Replacing Factor (TRF) and B Cell Differentiation Factor (BCDF) by secreting IgM.     

Propagation:ATCC complete growth medium:  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium,Catalog No. 30-2001.  

To make the complete growth medium, add he following components to the base medium:  fetal bovine serum to a final concentration of 10%.

Temperature 培養(yǎng)溫度 : 37.0°C 

Subculturing:Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. 

Start cultures at 5 x 10 exp5 cells/ml and maintain between 4 x 10 exp5 and 2 x 10 exp6 cells/ml.

Preservation:culture medium 95%; DMSO, 5% Related 

Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 

References 參考文獻 :1149: Saiki O, Ralph P. Clonal differences in response to T cell replacing factor (TRF) for IgM secretion and TRF receptors in a human B lymphoblast cell line.  Eur. J.Immunol. 13: 31-34, 1983. PubMed: 6339249

58055: Ralph P, et al. IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester.Immunol. Lett. 7: 17-23,1983. PubMed: 6605913