細(xì)胞形態(tài):上皮樣
生長(zhǎng)狀態(tài):貼壁生長(zhǎng)
ATCC Number:CCL-35?
相關(guān)疾?。赫?br />
器官來(lái)源:腎臟
數(shù)量:大量
年限:adult
運(yùn)輸方式:凍存運(yùn)輸
是否是腫瘤細(xì)胞:0
物種來(lái)源:其他
規(guī)格:0.2ml Designations: Pt K1 (NBL-3) [PTK1]
Depositors: ?KH Walen
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Potorous tridactylis
Morphology:epithelial
Source: Organ: kidney
Disease: normal
Cellular Products:keratin
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Virus Resistance:poliovirus 2
Age: adult
Gender: female
Comments:The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Rinse cell sheet twice with 0.25% trypsin, 0.03% EDTA (or Alsever's Trypsin Versene) solution, remove the trypsin solution, and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
References: 26188: . . Nature 194: 406, 1962.
26189: . . Cytogenetics 3: 19, 1964.