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生長狀態(tài)：懸浮生長，少量貼壁
器官來源：脾
數(shù)量：大量
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：CRL-2826?
運輸方式：凍存運輸
品系：CAF1
細胞形態(tài)：淋巴樣
免疫類型：mouse IgG1 kappa
規(guī)格：0.5mg Designations: Aw3.18.14
Depositors: ?ER Unanue
Isotype: mouse IgG1 kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, with some loosely adherent cells
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast


Source: Organ: spleen
Strain: CAF1
Cell Type: hybridoma: B lymphocyte; somatic cell hybrid
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1996
Comments:Animals were immunized with M12-I-Ak 48-62 cells [PubMed: 9208845]. Spleen cells were fused with P3X63Ag8 myeloma cells.The antibody recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecule I-Ak.
Propagation: ATCC complete growth medium: Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine that is modified by ATCC to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonates supplemented with 1.5 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, 116mg/L L-arginine HCl, 41 mg/L L-asparagine.H2O and 10% heat-inactivated fetal bovine serum
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Shake off the attached cells and transfer along with the floating cells into new flasks. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 3 X 10(4) viable cells/ml.
Interval: Maintain cell density between 2 X 10(4) and 2 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 14 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 30557: Dadaglio G, et al. Characterization and quantitation of peptide-MHC complexes produced from hen egg lysozyme using a monoclonal antibody. Immunity 6: 727-738, 1997. PubMed: 9208845