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平臺(tái)編號(hào)：bio-68205
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國際編號(hào)：CRL-1603?
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細(xì)胞信息： N1-S1 Fudr
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
N1-S1 Fudr
生長狀態(tài)：懸浮生長
品系：Sprague-Dawley
數(shù)量：大量
ATCC Number：CRL-1603?
相關(guān)疾?。浩渌膊?br /> 運(yùn)輸方式：凍存運(yùn)輸
器官來源：肝
是否是腫瘤細(xì)胞：0
物種來源：大鼠
規(guī)格：1mg Designations: N1-S1 Fudr
Depositors: ?JE Becker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:
Source: Organ: liver
Strain: Sprague-Dawley
Disease: hepatoma; Novikoff hepatoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments:The cells are resistant to 5-fluoro-2'-deoxyuridine (FUdR) at 0.05 mM and to 5-fluorouracil.
This line is derivative of N1-S1 (ATCC CRL-1604 ).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
Subculturing: Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 viable cells/ml and subculture at 1 X 10 exp6 cells/ml (about 3 days). Cells can be grown in Florence flasks on a rotary shaker at 180 to 190 RPM.
References: 1084: Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488
26107: Cory JG, et al. Evidence for role of purine nucleoside phosphorylase in sensitivity of Novikoff hepatoma cells to 5-fluorouracil. Adv. Enzyme Regul. 15: 153-166, 1976. PubMed: 197803
品系：Sprague-Dawley
數(shù)量：大量
ATCC Number：CRL-1603?
相關(guān)疾?。浩渌膊?br /> 運(yùn)輸方式：凍存運(yùn)輸
器官來源：肝
是否是腫瘤細(xì)胞：0
物種來源：大鼠
規(guī)格：1mg Designations: N1-S1 Fudr
Depositors: ?JE Becker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:
Source: Organ: liver
Strain: Sprague-Dawley
Disease: hepatoma; Novikoff hepatoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments:The cells are resistant to 5-fluoro-2'-deoxyuridine (FUdR) at 0.05 mM and to 5-fluorouracil.
This line is derivative of N1-S1 (ATCC CRL-1604 ).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
- 10% fetal bovine serum
- 0.05 mM FUdR
- 0.1% pluronic F68
Subculturing: Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 viable cells/ml and subculture at 1 X 10 exp6 cells/ml (about 3 days). Cells can be grown in Florence flasks on a rotary shaker at 180 to 190 RPM.
References: 1084: Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488
26107: Cory JG, et al. Evidence for role of purine nucleoside phosphorylase in sensitivity of Novikoff hepatoma cells to 5-fluorouracil. Adv. Enzyme Regul. 15: 153-166, 1976. PubMed: 197803
