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平臺(tái)編號(hào)：bio-68203
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國際編號(hào)：CRL-2018?
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細(xì)胞信息： SV40LT-SMC Clone HEP-SA
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
SV40LT-SMC Clone HEP-SA
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來源：大動(dòng)脈
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：大鼠
年限：3 months
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
品系：Sprague-Dawley
ATCC Number：CRL-2018?
組織來源：smooth muscle
細(xì)胞形態(tài)：成纖維樣
規(guī)格：48T Designations: SV40LT-SMC Clone HEP-SA
Depositors: ?CF Reilly, R McFall
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:fibroblast
Source: Organ: aorta
Strain: Sprague-Dawley
Tissue: smooth muscle
Cell Type: SV40 transfected
Cellular Products:alpha actin; large T antigen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 3 months
Comments:This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats.
The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen.
Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance).
Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin.
Large T antigen is detectable in the nuclei.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90% supplemented with 0.2 mg/ml G418, 90%; bovine calf serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove. Allow the cells to sit at 37C (or at room temperature) until the cells detach (usually 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2030
References: 23008: Reilly CF. Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin. J. Cell. Physiol. 142: 342-351, 1990. PubMed: 2154504
器官來源：大動(dòng)脈
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：大鼠
年限：3 months
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
品系：Sprague-Dawley
ATCC Number：CRL-2018?
組織來源：smooth muscle
細(xì)胞形態(tài)：成纖維樣
規(guī)格：48T Designations: SV40LT-SMC Clone HEP-SA
Depositors: ?CF Reilly, R McFall
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:fibroblast
Source: Organ: aorta
Strain: Sprague-Dawley
Tissue: smooth muscle
Cell Type: SV40 transfected
Cellular Products:alpha actin; large T antigen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 3 months
Comments:This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats.
The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen.
Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance).
Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin.
Large T antigen is detectable in the nuclei.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90% supplemented with 0.2 mg/ml G418, 90%; bovine calf serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove. Allow the cells to sit at 37C (or at room temperature) until the cells detach (usually 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2030
References: 23008: Reilly CF. Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin. J. Cell. Physiol. 142: 342-351, 1990. PubMed: 2154504
