運輸方式:凍存運輸
組織來源:connective tissue
數(shù)量:大量
是否是腫瘤細胞:0
物種來源:小鼠
ATCC Number:CCL-148?
相關(guān)疾?。豪w維肉瘤
細胞形態(tài):成纖維樣
生長狀態(tài):貼壁生長
規(guī)格:0.1ml Designations: HSDM1C1
Depositors: ?AH Tashjian
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Tissue: connective tissue
Disease: fibrosarcoma
Cellular Products:prostaglandin E2 (PGE2)
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Virus Resistance:poliovirus 1
Comments:Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Ham's F10 medium, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
References: 1029: Levine L, et al. Prostaglandin production by mouse fibrosarcoma cells in culture: inhibition by indomethacin and aspirin. Biochem. Biophys. Res. Commun. 47: 888-896, 1972. PubMed: 5063598
26011: . Calcium, parathyroid hormones and the calcitonins. Amsterdam: Excerpta Medica; 1972.
26012: . . Proc. Am. Assoc. Cancer Res. 3: 113, 1960.
26013: . . J. Dent. Res. 45: 490-499, 1966.