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HSDM1C1
    HSDM1C1
  • 平臺編號:bio-68198
  • 國際編號:CCL-148?
  • 細胞信息: HSDM1C1
  • 規(guī)格:frozen
  • 用途:ATCC原裝細胞
  • 服務(wù)費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

運輸方式:凍存運輸
組織來源:connective tissue
數(shù)量:大量
是否是腫瘤細胞:0
物種來源:小鼠
ATCC Number:CCL-148?
相關(guān)疾?。豪w維肉瘤
細胞形態(tài):成纖維樣
生長狀態(tài):貼壁生長
規(guī)格:0.1ml Designations: HSDM1C1
Depositors: ?AH Tashjian
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast


Source: Tissue: connective tissue
Disease: fibrosarcoma
Cellular Products:prostaglandin E2 (PGE2)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 1
Comments:Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Ham's F10 medium, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
References: 1029: Levine L, et al. Prostaglandin production by mouse fibrosarcoma cells in culture: inhibition by indomethacin and aspirin. Biochem. Biophys. Res. Commun. 47: 888-896, 1972. PubMed: 5063598
26011: . Calcium, parathyroid hormones and the calcitonins. Amsterdam: Excerpta Medica; 1972.
26012: . . Proc. Am. Assoc. Cancer Res. 3: 113, 1960.
26013: . . J. Dent. Res. 45: 490-499, 1966.

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