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數(shù)量：大量
細(xì)胞類型：T淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：TIB-233?
相關(guān)疾?。毫馨土?br /> 細(xì)胞形態(tài)：淋巴樣
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
品系：AKR/J
規(guī)格：0.1ml Designations: BW5147(T200-a)5.2
Depositors: ?R Hyman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Disease: lymphoma
Strain: AKR/J
Cell Type: T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:This line is a derivative of BW5147.G.1.4.OUAR.1 (ATCC CRL-1588 ).
The cells do not synthesize detectable amounts of the CD45 glycoprotein and do not express this molecule on the cell surface.
They are also negative for the CD45, T200, and Lea antigens.
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 to 8 X 10(4) viable cells/ml. Maintain cell density between 5 X 10(4) and 1 X 10(6) viable cells/ml. Do not exceed 5 X 10(5) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
parental cell line:ATCC CRL-1588
References: 22645: Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313