-
平臺(tái)編號(hào)：bio-68190
-
國(guó)際編號(hào)：CRL-2859?
-
細(xì)胞信息： CBRM1/1
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
CBRM1/1
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
品系：BALB/c
ATCC Number：CRL-2859?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
器官來(lái)源：脾
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
規(guī)格：0.1ml Designations: CBRM1/1
Depositors: ?TA Springer
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Strain: BALB/c
Organ: spleen
Cell Type: hybridoma: lymphoblast B lymphocyte; somatic cell hybrid
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [89838 ] [89839 ]
Isolation: Isolation date: November 19, 1991
Comments:Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580 ). Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. [Pubmed: 7679388] The monoclonal antibodies produced by these hybridomas will be useful in determining the function of Mac-1 subregions involved in ligand recognition. [Pubmed: 9614126].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ ml. Maintain cultures at a cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml.
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 89838: Lu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126
89839: Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388
免疫類型：IgG2b
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
品系：BALB/c
ATCC Number：CRL-2859?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
器官來(lái)源：脾
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
規(guī)格：0.1ml Designations: CBRM1/1
Depositors: ?TA Springer
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Strain: BALB/c
Organ: spleen
Cell Type: hybridoma: lymphoblast B lymphocyte; somatic cell hybrid
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [89838 ] [89839 ]
Isolation: Isolation date: November 19, 1991
Comments:Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580 ). Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. [Pubmed: 7679388] The monoclonal antibodies produced by these hybridomas will be useful in determining the function of Mac-1 subregions involved in ligand recognition. [Pubmed: 9614126].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ ml. Maintain cultures at a cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml.
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 89838: Lu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126
89839: Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388
