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SFR3-DR5
    SFR3-DR5
  • 平臺編號:bio-68189
  • 國際編號:HB-151?
  • 細胞信息: SFR3-DR5
  • 規(guī)格:frozen
  • 用途:ATCC原裝細胞
  • 服務(wù)費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

生長狀態(tài):懸浮生長
是否是腫瘤細胞:0
物種來源:大鼠
數(shù)量:大量
運輸方式:凍存運輸
細胞形態(tài):淋巴樣
免疫類型:IgG2b
ATCC Number:HB-151?
規(guī)格:100T Designations: SFR3-DR5
Depositors: ?SF Radka
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against HLA DR5
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with purified DR antigen from obtained from the Swei human lymphoblastoid cell line.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1001: Radka SF, et al. SFR3-DR5, a monoclonal antibody with HLA-DR5 specificity. J. Immunol. 130: 1863-1866, 1983. PubMed: 6187822

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