細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：褐鼠
器官來源：其他
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞形態(tài)：神經(jīng)元
數(shù)量：大量
ATCC Number：CRL-2768?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。浩渌膊?br />
規(guī)格：1ml Designations: RT4-D6P2T
Depositors: ?PI Patel
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus
Morphology:neuronal
Source: Organ: peripheral nervous system
Disease: Schwannoma
Cell Type: neuronal Schwann cell;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Comments:Products:
cyclic nucleotide phosphodiesterase 1 (Cnp1) (CNPase); positive [PubMed: 2824698]Laminin; positive [PubMed: 2824698]Myelin-associated glycoprotein (Mag); Protein and mRNA, negative [PubMed: 12210843] Myelin basic protein (Mbp); protein and mRNA, negative [PubMed: 12210843]Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); protein and mRNA, positive [PubMed: 12210843 peripheral myelin protein 22 (Pmp22) mRNA, positive; protein, negative [PubMed: 12210843]S100 calcium-binding protein, beta (neural) (S100b); protein, positive [PubMed: 12210843]Receptors expressed:
Nerve growth factor receptor (Ngfr) ; protein, negative; mRNA, positive [PubMed: 12210843]Comments:
RT4-D6P2T is an schwannoma cell line derived from a N-ethyl-N-nitrosourea (ENU) induced rat peripheral neurotumor, RT4. The tumor was grown in culture and a cell line RT4-AC was isolated that subsequently developed in a distinct cell type, RT4-D that produces glial specific S100. RT4-D was subcloned to produce RT4-D6 that was subcloned to produce RT-4-D6P2 and then finally subcloned to produce the D6P2T subclone [PubMed: 2824698].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 64424: Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843
71903: Bansal R, Pfeiffer SE. Regulated galactolipid synthesis and cell surface expression in Schwann cell line D6P2T. J. Neurochem. 49: 1902-1911, 1987. PubMed: 2824698
