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平臺(tái)編號(hào)：bio-68163
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國(guó)際編號(hào)：CRL-2470?
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細(xì)胞信息： I-11.15
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
I-11.15
年限：adult
數(shù)量：大量
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：CRL-2470?
器官來(lái)源：脾
細(xì)胞形態(tài)：?jiǎn)魏思?xì)胞/巨噬細(xì)胞
品系：C3H/HeJ
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：48T Designations: I-11.15
Depositors: ?WS Walker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:macrophage
Source: Organ: spleen
Strain: C3H/HeJ
Cell Type: macrophage
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:colony stimulating factor 1 (CSF-1R, CD115)
Antigen Expression:CD11b/CD18 (Mac-1) +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) + [39971 ]
Age: adult
Gender: female
Comments:This is an immortalized cell line derived from the progeny of individual splenic macrophage progenitor cells of an apparently normal adult mouse. [39971 ]
The cell line is dependent on colony stimulating factor 1 (CSF-1).
The cell line possesses many features of mature macrophages, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor. [39971 ]
This cell line, unlike I-13.35 (ATCC CRL-2471 ), does not have the constitutive ability to present antigen to Th1 helper T cells and it expresses much lower levels of MHC Class II antigens than I-13-35. [39972 ]
The cell line can be used to phenotype and characterize the role of splenic macrophages in the initiation of immune responses. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove 75% of the media. Scrape off the attached cells and transfer to new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum: ATCC 30-2020
References: 38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
39971: Wilson CM, et al. Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors. J. Immunol. Methods 137: 17-25, 1991. PubMed: 1707081
39972: McCormack JM, et al. Mouse splenic macrophage cell lines with different antigen-presenting activities for CD4+ helper T cell subsets and allogeneic CD8+ T cells. Cell. Immunol. 145: 359-371, 1992. PubMed: 1451184
數(shù)量：大量
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：CRL-2470?
器官來(lái)源：脾
細(xì)胞形態(tài)：?jiǎn)魏思?xì)胞/巨噬細(xì)胞
品系：C3H/HeJ
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：48T Designations: I-11.15
Depositors: ?WS Walker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:macrophage
Source: Organ: spleen
Strain: C3H/HeJ
Cell Type: macrophage
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:colony stimulating factor 1 (CSF-1R, CD115)
Antigen Expression:CD11b/CD18 (Mac-1) +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) + [39971 ]
Age: adult
Gender: female
Comments:This is an immortalized cell line derived from the progeny of individual splenic macrophage progenitor cells of an apparently normal adult mouse. [39971 ]
The cell line is dependent on colony stimulating factor 1 (CSF-1).
The cell line possesses many features of mature macrophages, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor. [39971 ]
This cell line, unlike I-13.35 (ATCC CRL-2471 ), does not have the constitutive ability to present antigen to Th1 helper T cells and it expresses much lower levels of MHC Class II antigens than I-13-35. [39972 ]
The cell line can be used to phenotype and characterize the role of splenic macrophages in the initiation of immune responses. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove 75% of the media. Scrape off the attached cells and transfer to new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum: ATCC 30-2020
References: 38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
39971: Wilson CM, et al. Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors. J. Immunol. Methods 137: 17-25, 1991. PubMed: 1707081
39972: McCormack JM, et al. Mouse splenic macrophage cell lines with different antigen-presenting activities for CD4+ helper T cell subsets and allogeneic CD8+ T cells. Cell. Immunol. 145: 359-371, 1992. PubMed: 1451184
