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年限：1 year infant
運輸方式：凍存運輸
ATCC Number：TIB-202?
相關(guān)疾?。浩渌膊?br /> 器官來源：外周血
細(xì)胞形態(tài)：單核細(xì)胞/巨噬細(xì)胞
細(xì)胞類型：其他細(xì)胞類型
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：懸浮生長
規(guī)格：0.5mg                                                                                                                                                                                                                                                                                                                                                          Designations: THP-1
         Depositors:                  ?S Tsuchiya
         Biosafety Level:1
         Shipped:        frozen
         Medium & Serum: See Propagation          Growth        Properties:suspension
         Organism:          Homo sapiens
         Morphology:monocyte
                   

         Source:          Organ: peripheral blood
         Disease: acute monocytic leukemia
         Cell Type:  monocyte;
         Cellular Products:lysozyme
         Permits/Forms:In addition to the MTA          mentioned above, other ATCC            and/or regulatory permits  may be required for the          transfer of this ATCC material. Anyone purchasing ATCC          material is ultimately responsible for obtaining the permits.          Please click here  for information regarding          the specific requirements for shipment to your location.
             Applications:transfection host
         Receptors:complement (C3), expressed          [58053 ]
Fc, expressed
         Antigen        Expression:HLA A2, A9, B5, DRw1, DRw2          [58053 ]
         DNA          Profile (STR):Amelogenin: X,Y
CSF1PO: 11,13          
D13S317: 13
D16S539: 11,12
D5S818: 11,12          
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
         vWA: 16
         Age:        1 year infant
         Gender:        male
         Comments:The cells are phagocytic (for both          latex beads and sensitized erythrocytes) and lack surface and          cytoplasmic immunoglobulin.
Monocytic differentiation          can be induced with the phorbol ester          12-O-tetradecanoylphorbol-13-acetate (TPA).
         Propagation:                   ATCC complete growth medium:  The base          medium for this cell line is ATCC-formulated RPMI-1640 Medium,          Catalog No. 30-2001.  To make the complete growth medium, add          the following components to the base medium:          2-mercaptoethanol to a final concentration of 0.05 mM; fetal          bovine serum to a final concentration of 10%.
         Atmosphere: air, 95%; carbon dioxide (CO2),          5%
         Temperature: 37.0°C
         Subculturing:                  Protocol: Cultures can be maintained by the          addition of fresh medium or replacement of medium.          Alternatively, cultures can be established by centrifugation          with subsequent resuspension at 2-4 X 10(5) viable cells/ml.          Subcultutre when cell concentration reaches 8X105) cells/ml.Do          not allow the cell concentration to exceed 1 X 10(6) cells/ml.
         Medium Renewal: Every 2 to 3 days
         Preservation:                  Freeze medium: Complete growth medium          supplemented with 5% (v/v) DMSO
         Storage temperature: liquid nitrogen vapor phase
         Doubling          Time: approximately 26 hrs
         Related        Products:purified RNA:ATCC TIB-202R
purified DNA:ATCC TIB-202D
Recommended medium (without          the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2020
Cell culture tested DMSO:          ATCC 4-X
         References:           22193: Tsuchiya S, et al. Induction of          maturation in cultured human monocytic leukemia cells by a          phorbol diester.  Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641
          22285: Skubitz KM, et al. Human          granulocyte surface molecules identified by murine monoclonal          antibodies.  J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543
          32286: Cuthbert JA, Lipsky PE. Regulation          of proliferation and Ras localization in transformed cells by          products of mevalonate metabolism.  Cancer Res. 57: 3498-3504,          1997. PubMed: 9270019
          32351: Huang S, et al. Adenovirus          interaction with distinct integrins mediates separate events          in cell entry and gene delivery to hematopoietic cells.  J.          Virol. 70: 4502-4508, 1996. PubMed: 8676475
          32395: Clark RA, et al. Tenascin supports          lymphocyte rolling.  J. Cell Biol. 137: 755-765, 1997. PubMed:            9151679
          32466: Hambleton J, et al. Activation of          c-Jun N-terminal kinase in bacterial          lipopolysaccharide-stimulated macrophages.  Proc. Natl. Acad.          Sci. USA 93: 2774-2778, 1996. PubMed: 8610116
          33031: Hsu HY, et al. Inhibition of          macrophage scavenger receptor activity by tumor necrosis          factor-alpha is transcriptionally and post-transcriptionally          regulated.  J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819
          33088: Lucas M, Mazzone T. Cell surface          proteoglycans modulate net synthesis and secretion of          macrophage apolipoprotein E.  J. Biol. Chem. 271: 13454-13460,          1996. PubMed: 8662812
          33134: Sando GN, et al. Induction of          ceramide glucosyltransferase activity in cultured human          keratinocytes.  J. Biol. Chem. 271: 22044-22051, 1996. PubMed:            8703011
          33141: Ollivier V, et al. Elevated cyclic          AMP inhibits NF-kappaB-mediated transcription in human          monocytic cells and endothelial cells.  J. Biol. Chem. 271:          20828-20835, 1996. PubMed: 8702838
          58053: Tsuchiya S, et al. Establishment          and characterization of a human acute monocytic leukemia cell          line (THP-1).  Int. J. Cancer 26: 171-176, 1980. PubMed: 6970727