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平臺(tái)編號(hào)：bio-68140
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國際編號(hào)：CRL-1704?
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細(xì)胞信息： WEHI-279
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
WEHI-279
生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM (surface); kappa light chain
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：小鼠
品系：NZC
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
ATCC Number：CRL-1704?
相關(guān)疾病：淋巴瘤
規(guī)格：0.2ml Designations: WEHI-279
Depositors: ?NL Warner, LL Lanier
Isotype: IgM (surface); kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: NZC
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Comments:Does not secrete immunoglobulin.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 5 x 10(4) to 1 x 10(5) viable cells/ml. Maintain cell density between 5 x 10(4) and 5 x 10(5) cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22846: Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906
26146: Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325
58054: Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM (surface); kappa light chain
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：小鼠
品系：NZC
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
ATCC Number：CRL-1704?
相關(guān)疾病：淋巴瘤
規(guī)格：0.2ml Designations: WEHI-279
Depositors: ?NL Warner, LL Lanier
Isotype: IgM (surface); kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: NZC
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Comments:Does not secrete immunoglobulin.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 5 x 10(4) to 1 x 10(5) viable cells/ml. Maintain cell density between 5 x 10(4) and 5 x 10(5) cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22846: Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906
26146: Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325
58054: Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803
