運輸方式：凍存運輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：小鼠
年限：adult
器官來源：淋巴結(jié)
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
生長狀態(tài)：懸浮生長
ATCC Number：TIB-224?
品系：AKR/J
規(guī)格：0.2ml Designations: D10.G4.1
Depositors: ?C Janeway, J Kaye, P Conrad
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Organ: lymph node
Strain: AKR/J
Cell Type: helper T lymphocyte (Th-2);
Cellular Products:interleukin 4 (interleukin-4, IL-4); interleukin 5 (interleukin-5, IL-5)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 1 (IL-1), expressed
interleukin 1 (IL-1)
Antigen Expression:H-2k
Age: adult
Gender: male
Comments:The cells are specific for egg white conalbumin (Sigma No. C0755) and alloreactive to I-A b, v.
They can be used to assay IL-1 because they proliferate minimally in response to Con A in the absence of IL-1 or feeder cells.
Supernatant media from Con A stimulated D10.G4.1 cells contain IL-4 and IL-5.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- 10% T-STIM with Con A (rat IL-2 culture supplement ) Becton Dickinson, Catalog No. 354115)
- 10% fetal bovine serum
- 0.05 mM 2-mercaptoethanol
- 10 pg/ml mouse IL-1 alpha (R&D Systems, Catalog No. 400ML)
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Subculture actively growing suspension cultures before they have reached 1 X 10(6) cells/ml or the IL-1 and IL-2 will be rapidly depleted and the cells will lose viability. Use inoculation densities of 2 X 10 (5) viable cells/ml.
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 5% (v/v) DMSO.
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1763: Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804
22166: . . Immunol. Rev. 81: 40, 1984.
22205: Kaye J, Janeway CA J. Induction of receptors for interleukin 2 requires T cell Ag:Ia receptor crosslinking and interleukin 1. Lymphokine Res. 3: 175-182, 1984. PubMed: 6436584
22359: Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524
22817: Kaye J, et al. Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158: 836-856, 1983. PubMed: 6193236
23435: Kaye J, Janeway CA Jr.. The Fab fragment of a directly activating monoclonal antibody that precipitates a disulfide-linked heterodimer from a helper T cell clone blocks activation by either allogeneic Ia or antigen and self-Ia. J. Exp. Med. 159: 1397-1412, 1984. PubMed: 6232337
23451: Kaye J, et al. Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J. Immunol. 133: 1339-1345, 1984. PubMed: 6235287
23452: Kaye J, Janeway CA Jr.. The alpha- and beta-subunits of a murine T cell antigen/Ia receptor have a molecular weight of 31,000 in the absence of N-linked glycosylation. J. Immunol. 133: 2291-2300, 1984. PubMed: 6237148
23470: Swain SL, et al. Role of antigen in the B cell response. Specific antigen and the lymphokine IL-5 synergize to drive B cell lymphoma proliferation and differentiation to Ig secretion. J. Immunol. 140: 4224-4230, 1988. PubMed: 2453572
