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生長狀態(tài)：混合型生長
品系：C57BL
細(xì)胞形態(tài)：其他
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：CRL-1642?
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
器官來源：肺
相關(guān)疾病：其他疾病
規(guī)格：0.5mg 

Designations 統(tǒng)一別名 : LL/2 (LLC1)         

Depositors:JS Bertram         

Biosafety Level 生物安全等級(jí) :1         

Shipped:frozen         

Medium & Serum: See Propagation          

Growth Properties:mixed, adherent and suspension         

Organism:Mus musculus         

Morphology:rounded - loosely attached or floating 

Source:          

Strain:  C57BL         

Organ: lung         

Disease: Lewis lung carcinoma 

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits  may be required for the transfer of this ATCC material. 

Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.

Please click here  for information regarding the specific requirements for shipment to your location.

Applications 用途 :Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma. 

Tested and found negative for ectromelia virus (mousepox). The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate. This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.         

Tumorigenic:Yes         

Antigen        

Expression:H-2b         

Comments 注釋 :This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents. Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma. The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate. The cells are reported to be highly tumorigenic, but weakly metastatic in mice.

The cells form multilayers in flasks without actually becoming confluent. Tested and found negative for ectromelia virus (mousepox). 

Propagation:ATCC complete growth medium:The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.  

To make the complete growth medium, add the following components to the base medium:fetal bovine serum to a final concentration of 10%. 

Temperature 培養(yǎng)溫度 : 37.0°C   

Subculturing:

Protocol: Subcultures are prepared by diluting the suspension 1:4 to 1:6. 

Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution.         

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended 

Medium Renewal: 2 to 3 times per week   

Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature 保藏溫度 : liquid nitrogen vapor phase 

Doubling Time: 21 hrs        

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020         

References 參考文獻(xiàn) :1091: Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139   

45212: Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function.J. Immunol. 163: 5020-5028, 1999. PubMed:10528207