![KU812F [KU-812-F]](/static/images/cells-no_picture.jpg)
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平臺編號：bio-68113
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國際編號：CRL-2101?
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細胞信息： KU812F [KU-812-F]
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
KU812F [KU-812-F]
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：人
數(shù)量：大量
運輸方式：凍存運輸
ATCC Number：CRL-2101?
相關疾?。浩渌膊?br /> 生長狀態(tài)：懸浮生長
年限：38 years adult
器官來源：外周血
細胞形態(tài)：原始粒細胞
規(guī)格：0.2ml Designations: KU812F [KU-812-F]
Depositors: ?K Kishi
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:myeloblast
Source: Organ: peripheral blood
Disease: chronic myelogenous leukemia
Cell Type: basophil;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 38 years adult
Gender: male
Comments:KU812F is a clone of KU812 (ATCC CRL-2099 ). The parent KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers [PubMed: 85212346].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml. Do not allow the cell concentration to exceed 3 x 10(6) cells/ml.
Medium Renewal: Add fresh medium every two to three days (depending on cell density).
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-2099
References: 44220: Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609
44221: Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed: 2460439
44222: Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048
是否是腫瘤細胞：0
物種來源：人
數(shù)量：大量
運輸方式：凍存運輸
ATCC Number：CRL-2101?
相關疾?。浩渌膊?br /> 生長狀態(tài)：懸浮生長
年限：38 years adult
器官來源：外周血
細胞形態(tài)：原始粒細胞
規(guī)格：0.2ml Designations: KU812F [KU-812-F]
Depositors: ?K Kishi
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:myeloblast
Source: Organ: peripheral blood
Disease: chronic myelogenous leukemia
Cell Type: basophil;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 38 years adult
Gender: male
Comments:KU812F is a clone of KU812 (ATCC CRL-2099 ). The parent KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers [PubMed: 85212346].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml. Do not allow the cell concentration to exceed 3 x 10(6) cells/ml.
Medium Renewal: Add fresh medium every two to three days (depending on cell density).
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-2099
References: 44220: Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609
44221: Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed: 2460439
44222: Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048
