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細胞形態(tài)：其他 

器官來源：皮膚 

運輸方式：凍存運輸 

品系：C57BL/6J 

是否是腫瘤細胞：0 

物種來源：小鼠 

ATCC Number：CRL-6475

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生長狀態(tài)：貼壁生長 

數(shù)量：大量 

規(guī)格：0.1ml                                                                                                                 Designations 統(tǒng)一別名 : B16-F10 

Biosafety Level 生物安全等級 :1 

Shipped 提供形式 :frozen 

Medium & Serum: See Propagation          

Growth Properties:adherent 

Organism:Mus musculus 

Morphology:mixture of spindle-shaped and epithelial-like cells 

Source:          

Organ: skin Strain:C57BL/6J 

Disease: melanoma Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits  may be required for the transfer of this ATCC material. 

Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.

Please click here for information regarding the specific requirements for shipment to your location. Applications:transfection host 

Propagation:ATCC complete growth medium:The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.  

To make the complete growth medium, add the following components to the base medium:fetal bovine serum to a final concentration of 10%. 

Temperature 培養(yǎng)溫度 : 37.0℃ 

Atmosphere 需氧情況 : air, 95%; carbon dioxide (CO2), 5% 

Subculturing: 

Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-  0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. 

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37?C. 

Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended 

Medium Renewal: Every 2 to 3 days 

Preservation:Freeze medium: culture medium, 95%; DMSO, 5% 

Storage temperature 保藏溫度 : liquid nitrogen vapor phase 

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 

References 參考文獻 :22151: Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo.Cancer Res. 35: 218-224, 1975. PubMed: 1109790 

22191: Fidler IJ, et al. Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A.Cancer Res.36: 3608-3615, 1976. PubMed: 953987 

22192: Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes.Cancer Res. 37: 3945-3956, 1977. PubMed: 908034 

22243: Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor.Science 197: 893-895, 1977. PubMed: 887927 

23224: Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells.  J. Natl. Cancer Inst. 60: 1217-1222, 1978.PubMed: 418183 

23362: . .  Nat. New Biol. 242: 148-149, 1973. 16173787: Li M, et al. Loss of intracisternal A-type retroviral particles in BL6 melanoma cells transfected with MHC class I genes. J.Gen. Virol. 77:2757-2765, 1996. PubMed: 8922469