細(xì)胞形態(tài)：其他
器官來(lái)源：皮膚
運(yùn)輸方式：凍存運(yùn)輸
品系：C57BL/6J
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：CRL-6475
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生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
規(guī)格：0.1ml Designations 統(tǒng)一別名 : B16-F10
Biosafety Level 生物安全等級(jí) :1
Shipped 提供形式 :frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Mus musculus
Morphology:mixture of spindle-shaped and epithelial-like cells
Source:
Organ: skin Strain:C57BL/6J
Disease: melanoma Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.
Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.
Please click here for information regarding the specific requirements for shipment to your location. Applications:transfection host
Propagation:ATCC complete growth medium:The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:fetal bovine serum to a final concentration of 10%.
Temperature 培養(yǎng)溫度 : 37.0℃
Atmosphere 需氧情況 : air, 95%; carbon dioxide (CO2), 5%
Subculturing:
Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature 保藏溫度 : liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
References 參考文獻(xiàn) :22151: Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo.Cancer Res. 35: 218-224, 1975. PubMed: 1109790
22191: Fidler IJ, et al. Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A.Cancer Res.36: 3608-3615, 1976. PubMed: 953987
22192: Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes.Cancer Res. 37: 3945-3956, 1977. PubMed: 908034
22243: Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor.Science 197: 893-895, 1977. PubMed: 887927
23224: Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells. J. Natl. Cancer Inst. 60: 1217-1222, 1978.PubMed: 418183
23362: . . Nat. New Biol. 242: 148-149, 1973. 16173787: Li M, et al. Loss of intracisternal A-type retroviral particles in BL6 melanoma cells transfected with MHC class I genes. J.Gen. Virol. 77:2757-2765, 1996. PubMed: 8922469
