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平臺(tái)編號(hào)：bio-68099
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國(guó)際編號(hào)：TIB-206™
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細(xì)胞信息： DAKIKI
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu) 說(shuō)明書(shū)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
DAKIKI
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgA; lambda light chain
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：TIB-206?
規(guī)格：0.2ml Designations: DAKIKI
Depositors: ?P Ralph
Isotype: IgA; lambda light chain
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Cell Type: lymphoblast; Epstein-Barr virus (EBV) transformed
Cellular Products:immunoglobulin; monoclonal antibody
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Surface IgA positive human lymphocytes were selected from peripheral blood and cervical lymph node lymphocytes of an African patient with nasopharyngeal carcinoma and subsequently transformed with EBV.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 1147: Steinitz M, Klein G. EBV-transformation of surface IgA-positive human lymphocytes. J. Immunol. 125: 194-196, 1980. PubMed: 6247394
58055: Ralph P, et al. IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester. Immunol. Lett. 7: 17-23, 1983. PubMed: 6605913
