是否是腫瘤細(xì)胞：0
物種來(lái)源：牛
品系：Holstein
ATCC Number：CRL-1390?
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來(lái)源：其他
運(yùn)輸方式：凍存運(yùn)輸
年限：newborn, calf
數(shù)量：大量
規(guī)格：0.1ml Designations: BT
Depositors: ?AW McClurkin
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Bos taurus
Morphology:
Source: Organ: turbinate
Strain: Holstein
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: newborn, calf
Comments:This line has been tested, and found negative for, Bovine viral diarrhea virus (BVDV). The cells should not be grown in medium containing serum of bovine origin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
References: 23135: McClurkin AW, et al. Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus. Arch. Virusforsch. 45: 285-289, 1974. PubMed: 4472511
28594: Bermudez-Cruz R, et al. Characterization of an abundant, unique 1.7-kilobase bovine herpesvirus 4 (BHV-4) late RNA and mapping of a BHV-4 IE2 transactivator-binding site in its promoter-regulatory region. J. Virol. 71: 527-538, 1997. PubMed: 8985381
