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MD2
    MD2
  • 平臺編號:bio-68072
  • 國際編號:HB-229?
  • 細(xì)胞信息: MD2
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
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  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

ATCC Number:HB-229?
細(xì)胞形態(tài):淋巴樣
免疫類型:IgM
生長狀態(tài):懸浮生長
運(yùn)輸方式:凍存運(yùn)輸
數(shù)量:大量
是否是腫瘤細(xì)胞:0
物種來源:大鼠
規(guī)格:0.1ml Designations: MD2
Depositors: ?RV Rouse
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with mouse thymic stromal tissue.
Spleen cells were fused with Sp2/0 myeloma cells.
In the mouse thymus, MD2 is specific for dendritic cells in the medulla.
The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.
It also reacts with epithelial elements in a variety of mouse tissues.
Propagation: ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 58334: Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413

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