亚日韩在线中文字幕亚洲,国产在线播放鲁啊鲁视频,张柏芝手扒性器全部图片,亚洲成无码电影在线观看

生長狀態(tài)：混合型生長
品系：C57BL/6
ATCC Number：CRL-11904?
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：小鼠
細(xì)胞形態(tài)：單核細(xì)胞/巨噬細(xì)胞
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
器官來源：骨髓
規(guī)格：0.5mg Designations: JAWSII
Depositors: ?ZymoGenetics, Inc.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:mixed, adherent and suspension
Organism: Mus musculus
Morphology:monocyte


Source: Organ: bone marrow
Strain: C57BL/6
Cell Type: immature dendritic cell; monocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Propagation: ATCC complete growth medium: Alpha minimum essential medium with ribonucleosides, deoxyribonucleosides, 4 mM L-glutamine, 1 mM sodium pyruvate and 5 ng/ml murine GM-CSF, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by transferring floating cells to a centrifuge tube.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Pool cells and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Note: This cell line grows very slowly.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Once per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
References: 38868: MacKay VL, Moore EE. Immortalized dendritic cells. US Patent 5,648,219 dated Jul 15 1997
47440: Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998