生長狀態(tài)：混合型生長
品系：C57BL/6
ATCC Number：CRL-11904?
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：小鼠
細胞形態(tài)：單核細胞/巨噬細胞
數(shù)量：大量
運輸方式：凍存運輸
器官來源：骨髓
規(guī)格：0.5mg Designations: JAWSII
Depositors: ?ZymoGenetics, Inc.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:mixed, adherent and suspension
Organism: Mus musculus
Morphology:monocyte
Source: Organ: bone marrow
Strain: C57BL/6
Cell Type: immature dendritic cell; monocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Propagation: ATCC complete growth medium: Alpha minimum essential medium with ribonucleosides, deoxyribonucleosides, 4 mM L-glutamine, 1 mM sodium pyruvate and 5 ng/ml murine GM-CSF, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by transferring floating cells to a centrifuge tube.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Pool cells and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Note: This cell line grows very slowly.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Once per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
References: 38868: MacKay VL, Moore EE. Immortalized dendritic cells. US Patent 5,648,219 dated Jul 15 1997
47440: Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998
