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描述；小鼠全能性胚胎干細胞，每支細胞量1×106

 

動物種別；小鼠，129S5/SvEvBrd（原129/SvEvBrd）品系

 

性別：雄

 

組織來源：小鼠胚胎內細胞團

 

形態(tài)：球形克隆

 

培養(yǎng)基和添加劑：mES完全培養(yǎng)液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES級FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（終濃度為1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

 

供應限制 A。僅供研究之用。

 

REFERENCE；Bader A, Al-Dubai H, Weitzer G. Leukemia Inhibitory Factor Modulates Cardiogenesis in Embryoid Bodies in Opposite Fashions. Circ. Res. 86: 787-794, 2000. PubMed: 10764413 Wolcik SM, Longley MA, Roop DR. Discovery of a novel murine keratin 6 (K6) isoform explains the absence of hair and nail defects in mice deficient for K6a and K6b. J. Cell Biol. 154: 619-630, 2001. PubMed: 11489919 Liu Y, et al. Sox17 is essential for the specification of cardiac mesoderm in embryonic stem cells. Proc Natl Acad Sci USA. 104(10): 3859-3864, 2007. PubMed: 17360443 Adams DJ, et al. A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction. Genomics 86(6): 753-758. 2005. PubMed: 16257172 Festing MF, et al. Revised nomenclature for strain 129 mice. Mamm. Genome. 10(8):836, 1999.PubMed: 10430671 Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391 Bradley A, Zheng B, Liu P. Thirteen years of manipulating the mouse genome: a personal history. Int. J. Dev. Biol. 42 (7): 943-950, 1998. PubMed: 9853825

 

中文名稱:小鼠胚胎干細胞AB2.2

 

提供形式：復蘇狀態(tài)