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細(xì)胞CELL>
示蹤細(xì)胞(GFP/LUC/RFP...)>
人結(jié)腸癌細(xì)胞(綠色熒光蛋白標(biāo)記)(DMEM) 產(chǎn)品信息
名稱SW620/GFP(人結(jié)腸癌細(xì)胞(綠色熒光蛋白標(biāo)記))(DMEM)(STR鑒定正確)
種屬人類
年齡(性別)男性,51歲
組織來源結(jié)直腸腺癌,來自轉(zhuǎn)移淋巴結(jié)
生長(zhǎng)特性貼壁細(xì)胞
細(xì)胞形態(tài)上皮細(xì)胞樣
生物安全等級(jí)1
生長(zhǎng)培養(yǎng)基DMEM+10%FBS+1%P/S
推薦傳代比例1:3-1:4
推薦換液頻率2~3次/周
倍增時(shí)間~20-26小時(shí)
凍存條件
凍存液:55%基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO
溫度:液氮
培養(yǎng)條件
氣相:空氣,95%;CO2,5%
溫度:37℃
致瘤性Yes,in nude mice(Tumors developed within 21 days at 100%frequency(5/5)in nude mice inoculated subcutaneously with 1×10^7 cells).
抗原表達(dá)情況Blood Type A;Rh+
基因表達(dá)情況Carcinoembryonic antigen(CEA)0.15 ng/10^6 cells/10 days;transforming growth factor alpha;matrilysin.The cells are negative for expression of CSAp(CSAp-)and colon antigen 3,negative.The cells are positive for keratin by immunoperoxidase staining.The line is positive for expression of c-myc,K-ras,H-ras,N-ras,Myb,sis and fos oncogenes.
保藏機(jī)構(gòu)ATCC;CCL-227 ECACC;87051203