M1/70.15.11.5.HL
TIB-128 ™
92/100
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105 Product Citations
Product category
Animal cells
Product type
Hybridoma
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell type
hybridoma: b lymphocyte
Tissue
Spleen
Applications
Immunology
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
General
Specific applications
The antibody precipitates two polypeptides of 190000 and 105000 daltons, binds to human monocytes, polymorphonuclear leukocytes and a small population of lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Characteristics
Growth properties
Suspension
Derivation
Spleen cells were fused with NS-1 myeloma cells.
The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC.
Genes expressed
immunoglobulin, monoclonal antibody, against mouse macrophage, granulocyte (Mac-1, alpha chain)
Isotype
Rat IgG2b
Comments
Animals were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes.
Spleen cells were fused with NS-1 myeloma cells.
Mac-1 is a mouse macrophage differentiation associated with type three complement receptor (CR3).
The antigen is expressed in large amounts on thioglycollate induced peritoneal exudate macrophages and in lesser quantities on neutrophilic granulocytes, blood monocytes.
8% of spleen cells, 44% of bone marrow cells and less than 0.3% of thymus cells react with the antibody.
The antibody precipitates two polypeptides of 190000 and 105000 daltons, binds to human monocytes, polymorphonuclear leukocytes and a small population of lymphocytes.
The antibody is capable of both natural killing and antibody dependent cellular cytotoxicity.
Tested and found negative for ectromelia virus (mousepox).
The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC.
Handling information
Quality control specifications
History
Legal disclaimers
