NU-DUL-1
CRL-2969™
NU-DUL-1 is a lymphocyte cell line that was isolated in 1985 from the peritoneal effusion of a 43-year-old, White male with lymphoma non-Burkitt's type. It can be used for immunology research.
Product category
Human cells
Organism
Homo sapiens, human
Cell type
lymphocyte
Morphology
early B-cell
Tissue
Peritoneal effusion
Disease
Lymphoma; Non-Burkitt's Type
Applications
3D cell culture
Immunology
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
General
Specific applications
The Nu-DUL-1 cell line is a valuable resource for studying the biological behavior of undifferentiated lymphoma cells.
The Nu-DUL-1 cell line will aid molecular and cellular studies designed to identify the biological significance of genomic rearrangements.
Characteristics
Growth properties
Suspension
Age
43 years
Ethnicity
White
Gender
Male
Karyotype
44, X (hypodiploid), +der(4)t(4;?)(q31;?). -6, -17, -18, -21, +2mar,der(1)t(1;?)(q32;?), del(4)(q25q31), del(5)(p12), del(6)(q21), del(8)(q24), der(11)t(11;?)(q21;?), der(12)t(12;?)(p13;?), der(14)t(14;?)(q13;), ter rea(19;21)(q13;q22)
Metastatic
Malignant cerebrospinal fluid
Antigen expression
LN-1+
LN-2+
LN-3+(anti-HLA-Dr)
BA-1+ (CD24)
CALLA (J5)(common ALL antigen; CD10)+
BA-2 - (CD9)
OKM1 - (myeloid marker)
Leu-7 - (natural killer T cell marker)
OKT-3 -, OKT-4 -, OKT-6 -, OKT-8 -, OKT-11 - (T cell markers)
BM-1 -, BM-2 -, BM-3 - ,BM-4 -, BM-5 - (myeloid markers)
EBNA - (Epstein-Barr Nuclear Antigen)
Genes expressed
sIg-
Comments
The depositor states that this cell line is negative for the Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
The NU-DUL-1 cell line represents a very unusual undifferentiated lymphoma.
The NU-DUL-1 cell line has a chromosome 14 translocation, but not from chromosome 11 or 18 and may represent a new variant.
This cell line is hypodiploid with the presumed loss of a Y chromosome.
NU-DUL-1 cells express markers of early B-cell differentiation.
Handling information
Unpacking and storage instructions
Check all containers for leakage or breakage.
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70°C. Storage at –70°C will result in loss of viability.
Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 7 minutes.
Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a new culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product.
Subculturing procedure
Cultures can be maintained by the addition of fresh medium. An inoculum of 2.0 x 105to 3.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:5 is recommended.
Medium renewal: every 2 to 3 days
Reagents for cryopreservation
Complete growth medium supplemented with 20% (v/v) fetal bovine serum and 10% DMSO (ATCC 4-X)
Quality control specifications
History
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