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平臺(tái)編號(hào)：bio-133149
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細(xì)胞信息： COLO-699
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：STR鑒定正確
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
COLO-699（人肺癌腺癌細(xì)胞）
COLO-699（人肺癌腺癌細(xì)胞）
產(chǎn)品類別：人源細(xì)胞系
生長(zhǎng)特性：貼壁生長(zhǎng)
細(xì)胞形態(tài)：上皮細(xì)胞樣
凍存條件：RPMI-1640+10%FBS
Synonyms: COLO-699（人肺癌腺癌細(xì)胞）COLO-699
Background: established from the pleural fluid of a 57-year-old woman with adenocarcinoma of the lung in 1986
Species: human (Homo sapiens)
Tissue: lung
Disease: lung adenocarcinoma
Gender: N/A
Morphology: adherent, epitheloid cells growing as monolayers
Growth Mode: N/A
Doubling Time: ca. 38 hours
DNA Profile: N/A
Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +; cells are HMB-45 -positive (this marker is highly associated with melanomas - hence the cells may be melanoma-derived)
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, MDH
Cytogenetics: human hypertriploid karyotype with 2% polyploidy - 70(62-72)<3n>XX, -X, +1, +3, -4, +6, +7, +7, +8, -9, -10, +12, -14, -16, -17, -17, +18, +20, der(2)t(2;10)(q27;q12), der(7)t(7;?)(p21;?)x2, i(10q), der(11)t(8;11)(q12;q14), idic(16q), der(21)t(21;?)(p12;?)x2 - a supernumerary small ring chromosome was present in 28% cells - corresponds to original karyotype with reinterpretation
Virus ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
