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首頁(yè)>細(xì)胞CELL>biobw自建細(xì)胞系>EPLC-272H(人肺癌鱗癌細(xì)胞)
EPLC-272H(人肺癌鱗癌細(xì)胞)
    EPLC-272H(人肺癌鱗癌細(xì)胞)
  • 平臺(tái)編號(hào):bio-133144
  • 細(xì)胞信息: EPLC-272H
  • 規(guī)格:1×10?cells/T25培養(yǎng)瓶
  • 用途:STR鑒定正確
  • 服務(wù)費(fèi)用:
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  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

 EPLC-272H(人肺癌鱗癌細(xì)胞)
產(chǎn)品類(lèi)別:人源細(xì)胞系
生長(zhǎng)特性:貼壁上皮樣細(xì)胞(無(wú)尖銳輪廓)以單層形式貼壁生長(zhǎng)
細(xì)胞形態(tài):貼壁
凍存條件:90%FBS+10%DMSO
Synonyms: EPLC-272HEPLC-272H(人肺癌鱗癌細(xì)胞)
Background: established from a surgical lung specimen of a 57-year-old Caucasian man who underwent surgery for undifferentiated squamous cell carcinoma of the lung (without having received prior radiation or chemotherapy) in 1986
Species: human (Homo sapiens)
Tissue: lung
Disease: epidermoid lung carcinoma
Gender: N/A 
Morphology: adherent epithelial-like cells (without sharp contours) growing adherently as monolayers
Growth Mode: N/A 
Doubling Time: ca. 60 hours
DNA Profile: N/A
Cell Line Authentication Service
Culture Medium: RPMI-1640+20%FBS
We strongly suggest to purchase the complete medium 
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, LDH, MDH
Cytogenetics: human hypotriploid karyotype with 12% polyploidy - 66(59-70)<3n>XX, -Y, -4, +7, +8, -18, -19, -20, -21, +6mar, add(1)(p32), del(2)(q33), der(2;11)(q10;q10), add(3)(p25), i(5p), i(5q), add(6)(q11), add(7)(q33)x2-3, add(7)(p15), del(8)(p12-21), del(10)(p14)x1-2, add(11)(q24)x2, add(12)(p11-12), add(13)(q33), add(14)(p12), i(15q), der(17)t(3;17)(p11;p12), add(19)(q13), add(21)(p12), dmx2 - matches published karyotype
Virus  ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV -
   

 

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