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平臺(tái)編號(hào)：bio-132034
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細(xì)胞信息： Hs766T/HS 766T
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：細(xì)胞系
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
人胰腺癌細(xì)胞Hs766T
細(xì)胞名稱：人胰腺癌細(xì)胞Hs766T
產(chǎn)品規(guī)格：T25培養(yǎng)瓶x1;1.5ml凍存管x2
細(xì)胞數(shù)量：1x10^6;1x10^6
保存溫度：37℃;-198℃
運(yùn)輸方式：常溫保溫運(yùn)輸；干冰運(yùn)輸
安全等級(jí)：1
用途限制：僅供科研2類
培養(yǎng)體系：90%DMEM+10%FBS+1%三抗
培養(yǎng)溫度：37℃
二氧化碳濃度：5%
簡(jiǎn)介：人胰腺癌細(xì)胞Hs766T取自64歲女性供體，部分文獻(xiàn)報(bào)道為46歲女性供體。該細(xì)胞為ATCC保藏。
注釋：Part of:Cancer Cell Line Encyclopedia(CCLE)project.
Part of:COSMIC cell lines project.
Part of:KuDOS 95 cell line panel.
Doubling time:6-7 days(PubMed=176412);30 hours(PubMed=25984343).
Microsatellite instability:Stable(MSS)(Sanger).
Omics:Array-based CGH.
Omics:Deep exome analysis.
Omics:Deep proteome analysis.
Omics:Deep RNAseq analysis.
Omics:DNA methylation analysis.
Omics:Metabolome analysis.
Omics:Proteome analysis by 2D-DE/MS.
Omics:shRNA library screening.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
Caution:Indicated to be from a 46 year old male patient in ATCC and from a 64 year old male patient in PubMed=283258 and PubMed=761205.
Caution:Incorrectly reported to have no KRAS mutation in PubMed=8026879 and PubMed=15367885.
Caution:TP53 mutation indicated incorrectly as being at c.542G>A in PubMed=1630814.
Misspelling:Hs-776-T;In Cosmic 873005,Cosmic 948380 and PubMed=10408907.
基因突變
Homozygous for KRAS p.Gln61His(c.183A>C)(CCLE;Cosmic-CLP).
Homozygous for SMAD4 deletion(PubMed=10408907;PubMed=15367885).
Has no TP53 mutation(PubMed=8026879;PubMed=15367885).
HLA信息
/
STR信息
Amelogenin X
CSF1PO 12
D2S1338 18,26
D3S1358 16
D5S818 11
D7S820 10
D8S1179 12,14
D13S317 8,11
D16S539 9,12(ATCC;CCRID;Cosmic-CLP)
9,11,12(PubMed=25877200)
D18S51 13
D19S433 15
D21S11 28
FGA 19,20
Penta D 9
Penta E 7,12
TH01 6,9.3
TPOX 8
vWA 18
參考文獻(xiàn)
PubMed=27259358;DOI=10.1074/mcp.M116.058313
Humphrey E.S.,Su S.-P.,Nagrial A.M.,Hochgrafe F.,Pajic M.,Lehrbach G.M.,Parton R.G.,Yap A.S.,Horvath L.G.,Chang D.K.,Biankin A.V.,Wu J.,Daly R.J.
Resolution of novel pancreatic ductal adenocarcinoma subtypes by global phosphotyrosine profiling.
Mol.Cell.Proteomics 15:2671-2685(2016)
PubMed=27397505;DOI=10.1016/j.cell.2016.06.017
Iorio F.,Knijnenburg T.A.,Vis D.J.,Bignell G.R.,Menden M.P.,Schubert M.,Aben N.,Goncalves E.,Barthorpe S.,Lightfoot H.,Cokelaer T.,Greninger P.,van Dyk E.,Chang H.,de Silva H.,Heyn H.,Deng X.,Egan R.K.,Liu Q.,Mironenko T.,Mitropoulos X.,Richardson L.,Wang J.,Zhang T.,Moran S.,Sayols S.,Soleimani M.,Tamborero D.,Lopez-Bigas N.,Ross-Macdonald P.,Esteller M.,Gray N.S.,Haber D.A.,Stratton M.R.,Benes C.H.,Wessels L.F.A.,Saez-Rodriguez J.,McDermott U.,Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)
PubMed=29444439;DOI=10.1016/j.celrep.2018.01.051
Yuan T.L.,Amzallag A.,Bagni R.,Yi M.,Afghani S.,Burgan W.,Fer N.,Strathern L.A.,Powell K.,Smith B.,Waters A.M.,Drubin D.,Thomson T.,Liao R.,Greninger P.,Stein G.T.,Murchie E.,Cortez E.,Egan R.K.,Procter L.,Bess M.,Cheng K.T.,Lee C.-S.,Lee L.C.,Fellmann C.,Stephens R.,Luo J.,Lowe S.W.,Benes C.H.,McCormick F.
Differential effector engagement by oncogenic KRAS.
Cell Rep.22:1889-1902(2018)
PubMed=30894373;DOI=10.1158/0008-5472.CAN-18-2747
Dutil J.,Chen Z.,Monteiro A.N.,Teer J.K.,Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res.79:1263-1273(2019)
PubMed=31068700;DOI=10.1038/s41586-019-1186-3
Ghandi M.,Huang F.W.,Jane-Valbuena J.,Kryukov G.V.,Lo C.C.,McDonald E.R.III,Barretina J.,Gelfand E.T.,Bielski C.M.,Li H.,Hu K.,Andreev-Drakhlin A.Y.,Kim J.,Hess J.M.,Haas B.J.,Aguet F.,Weir B.A.,Rothberg M.V.,Paolella B.R.,Lawrence M.S.,Akbani R.,Lu Y.,Tiv H.L.,Gokhale P.C.,de Weck A.,Mansour A.A.,Oh C.,Shih J.,Hadi K.,Rosen Y.,Bistline J.,Venkatesan K.,Reddy A.,Sonkin D.,Liu M.,Lehar J.,Korn J.M.,Porter D.A.,Jones M.D.,Golji J.,Caponigro G.,Taylor J.E.,Dunning C.M.,Creech A.L.,Warren A.C.,McFarland J.M.,Zamanighomi M.,Kauffmann A.,Stransky N.,Imielinski M.,Maruvka Y.E.,Cherniack A.D.,Tsherniak A.,Vazquez F.,Jaffe J.D.,Lane A.A.,Weinstock D.M.,Johannessen C.M.,Morrissey M.P.,Stegmeier F.,Schlegel R.,Hahn W.C.,Getz G.,Mills G.B.,Boehm J.S.,Golub T.R.,Garraway L.A.,Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
產(chǎn)品規(guī)格：T25培養(yǎng)瓶x1;1.5ml凍存管x2
細(xì)胞數(shù)量：1x10^6;1x10^6
保存溫度：37℃;-198℃
運(yùn)輸方式：常溫保溫運(yùn)輸；干冰運(yùn)輸
安全等級(jí)：1
用途限制：僅供科研2類
培養(yǎng)體系：90%DMEM+10%FBS+1%三抗
培養(yǎng)溫度：37℃
二氧化碳濃度：5%
簡(jiǎn)介：人胰腺癌細(xì)胞Hs766T取自64歲女性供體，部分文獻(xiàn)報(bào)道為46歲女性供體。該細(xì)胞為ATCC保藏。
注釋：Part of:Cancer Cell Line Encyclopedia(CCLE)project.
Part of:COSMIC cell lines project.
Part of:KuDOS 95 cell line panel.
Doubling time:6-7 days(PubMed=176412);30 hours(PubMed=25984343).
Microsatellite instability:Stable(MSS)(Sanger).
Omics:Array-based CGH.
Omics:Deep exome analysis.
Omics:Deep proteome analysis.
Omics:Deep RNAseq analysis.
Omics:DNA methylation analysis.
Omics:Metabolome analysis.
Omics:Proteome analysis by 2D-DE/MS.
Omics:shRNA library screening.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
Caution:Indicated to be from a 46 year old male patient in ATCC and from a 64 year old male patient in PubMed=283258 and PubMed=761205.
Caution:Incorrectly reported to have no KRAS mutation in PubMed=8026879 and PubMed=15367885.
Caution:TP53 mutation indicated incorrectly as being at c.542G>A in PubMed=1630814.
Misspelling:Hs-776-T;In Cosmic 873005,Cosmic 948380 and PubMed=10408907.
基因突變
Homozygous for KRAS p.Gln61His(c.183A>C)(CCLE;Cosmic-CLP).
Homozygous for SMAD4 deletion(PubMed=10408907;PubMed=15367885).
Has no TP53 mutation(PubMed=8026879;PubMed=15367885).
HLA信息
/
STR信息
Amelogenin X
CSF1PO 12
D2S1338 18,26
D3S1358 16
D5S818 11
D7S820 10
D8S1179 12,14
D13S317 8,11
D16S539 9,12(ATCC;CCRID;Cosmic-CLP)
9,11,12(PubMed=25877200)
D18S51 13
D19S433 15
D21S11 28
FGA 19,20
Penta D 9
Penta E 7,12
TH01 6,9.3
TPOX 8
vWA 18
參考文獻(xiàn)
PubMed=27259358;DOI=10.1074/mcp.M116.058313
Humphrey E.S.,Su S.-P.,Nagrial A.M.,Hochgrafe F.,Pajic M.,Lehrbach G.M.,Parton R.G.,Yap A.S.,Horvath L.G.,Chang D.K.,Biankin A.V.,Wu J.,Daly R.J.
Resolution of novel pancreatic ductal adenocarcinoma subtypes by global phosphotyrosine profiling.
Mol.Cell.Proteomics 15:2671-2685(2016)
PubMed=27397505;DOI=10.1016/j.cell.2016.06.017
Iorio F.,Knijnenburg T.A.,Vis D.J.,Bignell G.R.,Menden M.P.,Schubert M.,Aben N.,Goncalves E.,Barthorpe S.,Lightfoot H.,Cokelaer T.,Greninger P.,van Dyk E.,Chang H.,de Silva H.,Heyn H.,Deng X.,Egan R.K.,Liu Q.,Mironenko T.,Mitropoulos X.,Richardson L.,Wang J.,Zhang T.,Moran S.,Sayols S.,Soleimani M.,Tamborero D.,Lopez-Bigas N.,Ross-Macdonald P.,Esteller M.,Gray N.S.,Haber D.A.,Stratton M.R.,Benes C.H.,Wessels L.F.A.,Saez-Rodriguez J.,McDermott U.,Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)
PubMed=29444439;DOI=10.1016/j.celrep.2018.01.051
Yuan T.L.,Amzallag A.,Bagni R.,Yi M.,Afghani S.,Burgan W.,Fer N.,Strathern L.A.,Powell K.,Smith B.,Waters A.M.,Drubin D.,Thomson T.,Liao R.,Greninger P.,Stein G.T.,Murchie E.,Cortez E.,Egan R.K.,Procter L.,Bess M.,Cheng K.T.,Lee C.-S.,Lee L.C.,Fellmann C.,Stephens R.,Luo J.,Lowe S.W.,Benes C.H.,McCormick F.
Differential effector engagement by oncogenic KRAS.
Cell Rep.22:1889-1902(2018)
PubMed=30894373;DOI=10.1158/0008-5472.CAN-18-2747
Dutil J.,Chen Z.,Monteiro A.N.,Teer J.K.,Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res.79:1263-1273(2019)
PubMed=31068700;DOI=10.1038/s41586-019-1186-3
Ghandi M.,Huang F.W.,Jane-Valbuena J.,Kryukov G.V.,Lo C.C.,McDonald E.R.III,Barretina J.,Gelfand E.T.,Bielski C.M.,Li H.,Hu K.,Andreev-Drakhlin A.Y.,Kim J.,Hess J.M.,Haas B.J.,Aguet F.,Weir B.A.,Rothberg M.V.,Paolella B.R.,Lawrence M.S.,Akbani R.,Lu Y.,Tiv H.L.,Gokhale P.C.,de Weck A.,Mansour A.A.,Oh C.,Shih J.,Hadi K.,Rosen Y.,Bistline J.,Venkatesan K.,Reddy A.,Sonkin D.,Liu M.,Lehar J.,Korn J.M.,Porter D.A.,Jones M.D.,Golji J.,Caponigro G.,Taylor J.E.,Dunning C.M.,Creech A.L.,Warren A.C.,McFarland J.M.,Zamanighomi M.,Kauffmann A.,Stransky N.,Imielinski M.,Maruvka Y.E.,Cherniack A.D.,Tsherniak A.,Vazquez F.,Jaffe J.D.,Lane A.A.,Weinstock D.M.,Johannessen C.M.,Morrissey M.P.,Stegmeier F.,Schlegel R.,Hahn W.C.,Getz G.,Mills G.B.,Boehm J.S.,Golub T.R.,Garraway L.A.,Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
