ATCC Medium: 1249 Modified Baar’s Medium For Sulfate
Reducers
Component I
MgSO4………………………………2.0 g
Sodium Citrate……………………..5.0 g
CaSO4 x 2H2O……………………..1.0 g
NH4Cl……………………….………1.0 g
DI Water…………………………….400.0 ml
Component II
K2HPO4……………………………..0.5 g
DI Water…………………………….200.0 ml
Component III
Sodium Lactate….…………………3.5 g
Yeast Extract………………………..1.0 g
DI Water……………………………..400.0 ml
Each component is adjusted to pH 7.5 and autoclaved at 121ºC. The three
components are mixed aseptically and tubed under 97% N2 and 3% H2. Tube
while warm to exclude as much oxygen as possible.
Sterilize by Filtration
5% Ferrous Ammonium Sulfate, Fe(NH4)2(SO4)2; Biologist will add 0.1 ml to 5.0
ml of medium prior to inoculation.
*****Note: Ferrous Ammonium Sulfate solution is optional and should not be
added unless specified.
培養(yǎng)基配方(每升)
MgSO4 2.0g
Sodium Citrate(檸檬酸鈉) 5.0g
CaSO4·2H2O 1.0g
NH4Cl 1.0g
K2HPO4 0.5g
Yeast Extract(酵母提取物) 1.0g
pH7.5±0.2(25℃)
使用說(shuō)明
稱(chēng)取本品10.5g于1L蒸餾水或去離子水中,加熱溶解,于97%N2、3%H2環(huán)境下分裝,121℃高壓滅菌15分鐘,冷卻,每100ml培養(yǎng)基添加1支(35%乳酸鈉溶液1ml),備用。
如有需要,每5ml培養(yǎng)基可選擇性添加0.1ml過(guò)濾除菌的5%硫酸亞鐵銨溶液。