ATCC medium: 164 Cytophaga-Spirochete medium
Cytophaga:
NH4Cl ........................1.0 g
KH2PO4 .......................1.0 g
MgSO4 ........................0.5 g
CaCl2 ........................0.04 g
NaCl........................30.0 g
FeCl3 . 6H2O .................1.25 mg
NaHCO3 .......................5.0 g
Na2S . 9H2O ..................0.1 g
Yeast extract................1.0 g
Glucose......................1.0 g
Agar........................15.0 g
Distilled water..............1.0 L
Spirochete:
NH4Cl ........................1.0 g
KH2PO4 .......................1.0 g
MgSO4 ........................0.5 g
CaCl2 ........................0.04 g
FeCl3 . 6H2O .................1.25 mg
NaHCO3 .......................1.0 g
Na2S . 9H2O ..................0.5 g
Yeast extract................1.0 g
Glucose......................1.0 g
Agar........................15.0 g
Distilled water..............1.0 L
Sterilize glucose separately as a 10% solution. Aseptically add the
appropriate amount to obtain the final concentration indicated above.
Sterilize Na2S separately as a 10% solution. To 100 ml of Cytophaga
medium add 0.1 ml of the sterile Na2S solution, and to 100 ml of
Spirochete medium, add 0.5 ml of the sterile Na2S solution. Filtersterilize
NaHCO3 separately as a 5% solution. Add 10 ml and 2 ml to 100
ml of the Cytophaga and Spirochete media, respectively. For broth, use
0.1% agar as a detoxifier.